摘要
目的观察2型囊泡单胺转运体(VMAT2)靶点分子探针18F-FP-(+)-DTBZ评价2型糖尿病(T2DM)大鼠脑多巴胺能神经元的效果。方法将12只ZDF大鼠均分为T2DM组(fa/fa基因型)和对照组(fa/+基因型),每组6只。于大鼠3、6和12月龄时行18F-FP-(+)-DTBZ Micro-PET/CT显像,观察2组大鼠体质量、血糖及脑纹状体标准摄取比值(SUVR)变化。结果 T2DM组大鼠6月龄时体质量明显高于对照组(P<0.05),而3月和12月龄时2组体质量差异均无统计学意义(P均>0.05)。T2DM组大鼠3月龄时空腹血糖与对照组差异无统计学意义(P>0.05),而6月龄、12月龄时空腹血糖均明显高于对照组(P均<0.05)。T2DM组大鼠3月龄、6月龄时脑纹状体SUVR与对照组差异均无统计学意义(P均>0.05),12月龄时明显低于对照组(P<0.05)。结论 2型糖尿病可致大鼠脑纹状体VMAT2表达降低,进而引起脑多巴胺能神经元功能异常。
Objective To observe the effect of 18 F-FP-(+)-DTBZ,vesicular monoamine transporter 2(VMAT2)molecular probe for evaluating dopaminergic neurons in type 2 diabetes mellitus(T2DM)rats.Methods Twelve ZDF rats were equally divided into T2DM group(fa/fa)and the control group(fa/+)(each n=6).18 F-FP-(+)-DTBZ Micro-PET/CT imaging was performed when the rats being 3,6 and 12-month old in both groups to observe the changes of rats'mass,glucose and the standardized uptake value ratio(SUVR)in the striatum of brains.Results The rats'mass of those being 6-month old in T2DM group was significantly higher than that in control group(P<0.05),while there was no significant difference between groups for rats being 3 and 12-month old(both P>0.05).The fasting blood glucose of rats being 3-month old was not significant different between groups(P>0.05),while of rats being 6 and 12-month old were significantly higher in T2DM group than in control group(both P<0.05).The striatal SUVR in T2DM group was significantly lower than that of control group for rats being 12-month old(P<0.05),but there was no significant difference between groups for rats being 3 and 6-month old(both P>0.05).Conclusion T2DM could result decrease of VMAT2 expression in the striatum of rat's brain,which might lead to abnormal function of brain dopaminergic neurons.
作者
姜东朗
潘智伟
肖见飞
黄琪
任树华
华逢春
管一晖
谢芳
HUA Fengchun;GUAN Yihui;XIE Fang(PET Center,the Huashan Hospital Affiliated of Fudan University,Shanghai 200235,China)
出处
《中国医学影像技术》
CSCD
北大核心
2020年第12期1761-1765,共5页
Chinese Journal of Medical Imaging Technology
基金
国家自然科学基金(81801752、81571345)
上海市市级科技重大专项(2018SHZDZX01)。
关键词
糖尿病
帕金森病
正电子发射断层显像
多巴胺
单胺转运蛋白体
diabetes mellitus
Parkinson disease
positron-emission tomography
dopamine
monoamine transporter
