摘要
针对石墨烯材料存在活性基团少和不可逆相互叠堆的缺陷,以及难以制备成球这一问题,利用微流控装置控制微球形貌和尺寸,通过酰胺化反应对微球结构进行固化,表面改性后获得石墨烯/壳聚糖复合微球(G/CS microsphere)吸附剂。通过光学显微镜和扫描电子显微镜(scanning electron microscope,SEM)观察,证实微球具有较好的球形度,颗粒尺寸约为200μm。随后,以牛血清白蛋白(bovine albumin,BSA)和血红蛋白(hemoglobin,Hb)为分离对象,考察了新型吸附微球的静态、动态吸附行为。结果表明,复合微球对模型蛋白质的吸附与其等电点密切相关,吸附行为符合Langmuir吸附等温线模型及二级动力学模型,最大吸附容量分别为76.01 mg/g(BSA pH=5.8)和189.21 mg/g(Hb pH=6.8)。
For the problems of irreversible overlapping and difficult covalent grafting of graphene materials,a microfluidic device was used to prepare covalently grafted chitosan microspheres from graphene.After surface modification,graphene/chitosan composite microspheres were obtained.According to the dynamic adsorption law,the effects of pH,adsorption thermodynamics,adsorption kinetics were studied.The results showed that the maximum adsorption capacity of BSA was 76.01 mg/g(pH=5.8)and of hemoglobin was 189.21 mg/g(pH=6.8),respectively.The adsorption process conforms to the Langmuir adsorption isotherm model and the second-order kinetic model,and belongs to the single-molecular physical adsorption.
作者
艾浩
王文慧
杜开峰
AI Hao;WANG Wenhui;DU Kaifeng(School of Chemical Engineering, Sichuan University, Chengdu 610065, China)
出处
《中国科技论文》
CAS
北大核心
2020年第12期1352-1358,1370,共8页
China Sciencepaper
基金
国家自然科学基金资助项目(21676170)。
关键词
吸附
石墨烯/壳聚糖复合微球
牛血清白蛋白
血红蛋白
adsorption
graphene/chitosan composite microsphere
bovine albumin(BSA)
hemoglobin(Hb)
