摘要
目的:探讨肝癌中miR-1288表达与患者血清AFP、临床特征及预后的相关性。方法:纳入2012年1月至2014年12月于我院进行手术的肝癌患者100例,留取每位肝癌和癌旁组织各1例。QRT-PCR法检测组织中miR-1288的相对表达量;放射免疫法检测血清AFP含量;对患者进行术后随访了解预后;Pearson分析miR-1288与患者血清AFP相关性;分析miR-1288与患者临床特征的关系;Logistic分析miR-1288表达的影响因素;Kaplan-Meier分析肝癌组织中miR-1288表达与预后的相关性。结果:miR-1288在肝癌组织中的表达量较肝癌旁组织显著升高(P<0.05);肝癌患者血清中AFP的含量与肝癌组织中miR-1288的表达水平正相关关系(P<0.05);肝癌组织中miR-1288的表达与患者性别、年龄无明显相关性(均P>0.05),但与肝癌病程、肿瘤分化程度、淋巴结是否发生远处转移、肝癌TNM分期和肝癌病灶数目均存在明显相关性(均P<0.05);肝癌患者的肿瘤分化程度低、发生淋巴结远处转移、TNM分期大均是miR-1288高表达的危险因素(均P<0.05);miR-1288低表达的肝癌患者手术后生存时间显著高于miR-1288高表达患者(P<0.05)。结论:肝癌中miR-1288表达与AFP含量呈正相关关系,且能影响患者的部分临床特征及预后水平。
Objective:To explore the relationship between miR-1288 expression and serum AFP,clinical features and prognosis in hepatocellular carcinoma(HCC)patients.Methods:A total of 100 HCC patients who underwent surgery in our hospital from January 2012 to December 2014 were enrolled,and one case of liver cancer and para-cancerous tissue from each patient was collected.The expression of miR-1288 in HCC and para-cancerous tissues was detected by QRT-PCR.The content of serum AFP was detected by radioimmunoassay.All patients were followed up after operation to determine their prognosis.The correlation of miR-1288 with AFP and clinical characteristics was evaluated.The factors influencing miR-1288 expression were examined by Logistic analysis,and the correlation between miR-1288 in HCC tissues and prognosis of patients was analyzed by Kaplan-Meier.Results:The expression of miR-1288 in HCC tissues was significantly increased compared with adjacent tissues(P<0.05).The level of AFP in serum of patients with liver cancer was positively correlated with the expression level of miR-1288 in HCC tissues(P<0.05).The expression of miR-1288 in HCC tissues was not correlated with gender nor age of patients(P>0.05),but it was correlated with the course of liver cancer,tumor differentiation,distant metastasis of lymph nodes,TNM stage and the number of liver cancer lesions(P<0.05).The low degree of tumor differentiation,lymph node metastasis and high TNM stage were the risk factors for over-expression of miR-1288(P<0.05).The survival time of HCC patients with low miR-1288 expression was significantly higher than the patients with high miR-1288 expression(P<0.05).Conclusion:The expression of miR-1288 in HCC patients is positively correlated with AFP content,and it can affect partial clinical features and prognosis of patients.
作者
马吉德
李富辉
金兆鑫
毕文忠
韩玉梅
Ma Jide;Li Fuhui;Jin Zhaoxin;Bi Wenzhong;Han Yumei(The Fifth People's Hospital of Qinghai Province,Xining 810000,China;Qinghai Provincial Tibetan Hospital,Xining 810000;The Affiliated Hospital of Qinghai University,Xining 810000,China)
出处
《广西医科大学学报》
CAS
2020年第12期2230-2235,共6页
Journal of Guangxi Medical University
