摘要
Ten phenolic compounds(1a/1b,2–9)including a pair of enantiomers(1a/1b),along with eight analogues(2–9)were isolated from the fruit of Crataegus pinnatifida.Enantiomers 1a/1b were separated successfully by chiral chromatographic column.Their structures were established by comprehensive spectroscopic analyses,and the absolute configurations of enantiomers were determined by comparison between the experimental and calculated electronic circular dichroism(ECD)spectra.In addition,all isolates were investigated for their neuroprotective effects against H2O2–induced oxidative stress in human neuroblastoma SH-SY5Y cells.It was found that enantiomers 1a and 1b displayed significant neuroprotective activities but no enantioselectivity.In addition,compounds 3–6 showed obvious neuroprotective effects at different concentrations,while compound 8 exhibited potential neuroprotective effect at higher concentration(50μmol/L).
基金
This work was supported by Career Development Support Plan for Young and Middle-aged Teachers in Shenyang Pharmaceutical University(ZQN2018006)
the Project of Innovation Team Foundation(LT2015027).
