摘要
为探究乳双歧杆菌BB-12(BB-12)及植物乳杆菌P-8(P-8)对抑郁行为和肠道菌群的影响,本文构建了慢性温和不可预知性(CUMS)抑郁模型,分别采用BB-12及P-8对其进行为期6周的灌胃治疗。灌胃前、后,分别进行行为学测试;治疗结束后,采集粪便样本进行16S rRNA V4-V5区测序。结果显示:与CUMS组相比,2株益生菌均能显著缓解慢性压力诱导的抑郁行为(糖水消耗率及旷场得分显著增加,暗箱中所处时间显著减少),BB-12和P-8组的菌群结构显著偏离CUMS组;与健康对照组(HC)相比,CUMS组中OTU556,OTU194和OTU99的丰度显著升高,而OTU198的丰度显著降低,BB-12组显著降低了OTU556,OTU194和OTU99的丰度,P-8组显著调节了OTU556,OTU194,OTU99和OTU198的丰度;PICRUSt分析显示,与HC组相比,CUMS组肠道微生物的多种功能发生紊乱,2株益生菌仅能一定程度上缓解功能紊乱。研究表明,益生菌可通过调节肠道菌群缓解慢性压力诱导的抑郁相关症状。
This study aims at evaluating the effects of Bifidobacterium Lactis BB-12 and Lactobacillus plantarum P-8 on the behaviors and gut microbiota in a depression model induced by chronic unpredictable mild stress(CUMS).A depressed rat model was established by CUMS combined with separation.After 8 weeks,depressed model rats were treated with Bifidobacterium lactis BB-12 and Lactobacillus plantarum P-8 for six weeks by gavage.Additionally,healthy control group(HC)and model control group(CUMS)were given the same volume of sterile water.Behavioral tests were performed before and after gavage.After the end of the treatment,fecal samples were collected for 16S rRNA V4-V5 regions high-throughput sequencing.Results showed that two probiotics improved CUMS-induced depressed behaviors(including attenuated the decreased sucrose preference in the sucrose preference test and developed the activity and exploration of CUMS rat in the open field test,as well as in the light/dark test,pronounced decreased the time of CUMS rat spent in the dark zone)compared with CUMS group.Nonmetric Multidimensional Scaling(NMDS)revealed that probiotic supplement groups significantly modified the overall structure of the fecal bacterial community when compared to control groups(HC and CUMS).Furthermore,two prebiotics also resulted in altering specific taxonomic compared with the control groups,including Bifidobacterium Lactis BB-12 decreased the levels of OTU556,OTU194 and OTU99 while Lactobacillus plantarum P-8 reversed the changes of OTU556,OTU194,OTU99 and OTU198.PICRUSt analysis revealed that a number of functions about intestinal microflora were disturbed by CUMS,and the two probiotics can only relieve dysfunction to a certain degree.Taken together,these results indicate an anti-depressant effect of Bifidobacterium Lactis BB-12 and Lactobacillus plantarum P-8 in rats subjected to CUMS and that this effect could be due to regulating the structure,composition and function of gut microflora.
作者
屈婉
刘硕
马晨
闫鹤
Qu Wan;Liu Shuo;Ma Chen;Yan He(School of Food Science and Engineering,South China University of Technology,Guangzhou 510640;Infinitus(China)Co.,Ltd.,Guangzhou 510627;Beijing Scitop Bio-Tech Shareholding Co.,Ltd.,Beijing 101407)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2020年第12期114-121,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
中央高校基本科研业务费项目(D2170320)。
关键词
益生菌
CUMS抑郁模型
肠道菌群
probiotic
chronic unpredictable mild stress depressed model
gut microbiota
