摘要
目的:利用斑马鱼模型探讨通过食物提供充足营养的情况下,饮水中的矿物元素是否对青春期机体生长发育、脂代谢和抗氧化能力发挥积极作用。方法:将21 dpf(受精后天数)的斑马鱼幼鱼随机分为养鱼水组(FWG,对照组)、自来水组(TWG)、天然水组(NWG)和纯净水组(PWG),每组饲喂同样的食物,每日记录死亡尾数直至90 dpf。斑马鱼被麻醉处死后,测量其体重、体长及全鱼矿物元素、TC、TG、HDL-C、LDL-C、MDA水平和SOD活性,并分析水中的矿物元素与所测指标的相关性。结果:PWG组斑马鱼体内钙和锌含量、存活率、体重、体长显著下降;NWG组斑马鱼HDL-C水平显著升高,TG、MDA水平显著降低;相关分析表明,斑马鱼体长与水中钙、镁水平呈显著正相关,TG水平与水中钙、镁、锌水平呈显著负相关。结论:天然水因适宜的pH值和DO、适量的矿物元素水平以及合理的钙镁比,在3种常见饮水中最适合青春期斑马鱼机体的健康发育,而纯净水则因缺乏钙、镁离子而不利于其机体的生长。
Objective:To determine whether mineral elements in water show positive effects on developmental behaviors,lipid metabolism and antioxidant capacity of the body when food can provide with adequate nutrition by use of juvenile zebrafish.21 dpf(days after fertilization)juvenile zebrafish were randomly divided into four groups,fish water group(FWG,control group),tap water group(TWG),natural water group(NWG),and purified water group(PWG).Zebrafish were raised in corresponding water with the same feed,the number of deaths were recorded every day up to 90 dpf.After the zebrafish were put to death by anesthesia,body weight,body length and whole body mineral levels,TC,TG,HDL-C,LDL-C,MDA levels and SOD activity were measured,and the correlation were analysed.Results:Zebrafish in PWG showed significantly decreased calcium and zinc levels,survival rate,body weight and body length;zebrafish in NWG showed significantly increased HDL-C levels and significantly decreased TG,MDA levels;the correlation analysis showed that body length of zebrafish were significantly positively correlated with calcium and magnesium levels in water,TG levels were significantly negatively correlated with calcium,magnesium and zinc levels in water.Conclusion:Natural water,which has suitable pH,DO levels and proper amount of minerals together with reasonable Ca:Mg ratio,is the most suitable for body development,while purified water seems not suitable due to its lacking of calcium,magnesium.
作者
常成
陈艺煊
曹菲薇
陈娟
郑炀凡
朱加进
Chang Cheng;Chen Yixuan;Cao Feiwei;Chen Juan;Zheng Yangfan;Zhu Jiajin(College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310000)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2020年第12期103-113,共11页
Journal of Chinese Institute Of Food Science and Technology
关键词
青春期
饮水
矿物元素
脂代谢
斑马鱼
adolescence
drinking water
mineral elements
lipid metabolism
zebrafish(Danio rerio)
