摘要
目的:筛选合适的大孔树脂以富集苦丁茶中多酚类化合物,为苦丁茶的深层次研究提供参考。方法:采用3种抗氧化能力测定法综合评价苦丁茶多酚的体外抗氧化能力。研究大孔树脂对苦丁茶多酚类物质的静态吸附、解吸行为、动力学和热力学。结果:苦丁茶多酚是一种良好的天然抗氧化剂。AB-8、HP-20两种树脂对苦丁茶多酚的吸附遵循准二级动力学模型,受边界扩散、粒子内扩散和表面吸附相互作用的影响。AB-8、HP-20树脂分别符合Freundlich方程(r2=0.989,8)、Langmuir方程(r2=0.982,5)。35℃、30℃分别为AB-8、HP-20树脂吸附苦丁茶多酚的最佳温度。结论:所建立的方案可作为从特有药用植物中分离多酚的参考,并可扩大苦丁茶作为食品工业和制药的生物活性剂的应用。
Objective: To screen suitable macroporous resins to enrich the polyphenolic compounds in Kudingcha, and to provide references for in-depth research on Kudingcha. Methods: Three antioxidant capacity determination methods were used to comprehensively evaluate in vitro antioxidant capacity of Kudingcha polyphenols. The static adsorption, desorption behavior, kinetics and thermodynamics of polyphenols by macroporous resin were studied. Results: Kudingcha polyphenol is a good natural antioxidant. The adsorption of kudingcha polyphenols by AB-8 and HP-20 resins follows a quasi-second-order kinetic model and is affected by boundary diffusion, intraparticle diffusion and surface adsorption interaction. AB-8 and HP-20 resins comply with Freundlich equation(r2=0.989,8) and Langmuir equation(r2=0.982,5), respectively. It found that the optimum temperature for adsorption of kudingcha polyphenols by AB-8 was 35 °C, and 30 °C for HP-20. Conclusion: The established protocol can be used as a reference for the isolation of polyphenols from peculiar medicinal plants, and can expand the application of Kudingcha as a bioactive agent in the food industry and pharmacy.
作者
赵露
尹国利
邹成梅
胡婷
ZHAO Lu;YIN Guo-li;ZOU Cheng-mei;HU Ting(School of Environmental Ecology and Bioengineering,Wuhan Institute of Technology,Wuhan Hubei 430205,China)
出处
《中医药导报》
2020年第16期39-44,共6页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(21702156)
武汉工程大学第十一届研究生教育创新基金(CX2019201)。
关键词
苦丁茶
大叶冬青
多酚
大孔树脂
抗氧化
Kudingcha
Daye Dongqing(Ilex latifolia)
polyphenols
macroporous resin
antioxidant
