摘要
热休克蛋白70(Heat Shock Protein 70,HSP70)与生物体抗逆抗胁迫能力密切相关,其在生物体内发挥着十分重要的作用。本研究以杂交黄颡鱼(黄颡鱼Pelteobagrus fulvidraco♀×瓦氏黄颡鱼P.vachelli♂)为对象,得到735 bp的hsp70 cDNA核心序列,并进行了生物信息学分析;运用实时荧光定量PCR方法检测hsp70基因mRNA在不同组织中的相对表达量。结果显示,hsp70基因在肝脏、鳃、脑、肌肉4个组织中均有表达,其中,正常条件下,在肝脏的表达量显著高于在脑、鳃和肌肉的表达量(P<0.05);在20℃(常温组)、25℃、28℃和31℃4个温度条件下,杂交黄颡鱼hsp70基因在肝脏、鳃、脑和肌肉组织中的表达量总体上随温度升高呈上升趋势,其中,28℃和31℃下,hsp70基因在鳃中的表达量显著高于其他组织(P<0.05),表明鳃为杂交黄颡鱼温度应激的敏感组织,可能是作为呼吸代谢重要器官的鳃在温度急剧变化情况下的一种机体保护策略。
Heat shock protein 70(HSP70)is closely related to the stress resistance and anti-stress ability of organisms,where it plays a crucial role.In the present study,a 735 bp core cDNA sequence of the hsp70 gene isolated from the liver tissue of Pelteobagrus fulvidraco ♀×P.vachelli ♂ was amplified using reverse transcription polymerase chain reaction(RT-PCR),and the bioinformatics was analyzed.The relative mRNA expression of the hsp70 gene in different tissues was tested using quantitative real-time PCR(qPCR),which revealed that hsp70 mRNA was found in all tested tissues,including liver,gill,brain,and muscle,and hsp70 mRNA expression levels in liver tissue were significantly different from that in brain,gill,and muscle tissue(P<0.05).At 20℃(control),25℃,28℃,and 31℃,the hsp70 gene expression in liver,gill,brain,muscle tissue generally increased with increasing temperature.The hsp70 gene expression level in gill tissue at 28℃ and 31℃ was significantly higher than that in other tissues(P<0.05),which indicated that gill tissue was sensitive to heat stress in the hybrid.It was suggested that this may be an organism protection strategy for gill tissue,which is important for respiratory metabolism under extreme changes in temperature.
作者
朱凌威
张朝阳
刘钊
李良玉
唐洪
赵仲孟
陈雨薇
武佳韵
黄小丽
杨世勇
ZHU Lingwei;ZHANG Chaoyang;LIU Zhao;LI Liangyu;TANG Hong;ZHAO Zhongmeng;CHEN Yuwei;WU Jiayun;HUANG Xiaoli;YANG Shiyong(College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611130;College of Life Science,Sichuan Agricultural University,Ya’an 625014;Fisheries Research Institute,Chengdu Academy of Agricultural and Forestry Sciences,Chengdu 611130)
出处
《渔业科学进展》
CSCD
北大核心
2021年第1期47-55,共9页
Progress in Fishery Sciences
基金
2020年农业农村部农业重大技术协同推广计划试点四川省稻渔综合种养关键技术集成与推广应用项目(川农函[2020]184号)
四川省科技厅应用基础研究项目(2019YJ0515)
四川省大学生创新训练项目(201610626056)
四川农业大学双支计划项目(1921993244)共同资助。
关键词
高温应激
黄颡鱼
HSP70基因
组织表达
Heat stress
Pelteobagrus fulvidraco
hsp70 gene
Tissue expression
