摘要
目的分析PCR-反向点杂交法耐药基因检测和BD960结核分枝杆菌药物敏感性试验在结核分枝杆菌耐药性方面的差异,并探讨其临床应用价值。方法收集356例昆明市第三人民医院结核科住院患者的痰和灌洗液标本,分别用2种方法检测异烟肼(H)、利福平(R)、链霉素(S)、乙胺丁醇(E)的药物敏感性,结果通过校正卡方检验对比分析。结果 2种检测方法对单耐药、多耐药、耐多药结核菌检测,在99%的置信区间,校正χ2均是P <0.01。结论可认为2种方法对3种耐药的检测有差异性。结核菌药敏试验耐多药PCR-反向点杂交法优于BD960检测;单耐药、多耐药BD960药敏优于PCR-反向点杂交法。
Objective To analyze the difference between PCR-reverse dot hybridization drug resistance gene detection and BD960 Mycobacterium tuberculosis drug sensitivity test in identifying drug resistance of Mycobacterium tuberculosis,and to discuss its clinical efficacy.Methods The drug sensitivity of isoniazid(H),rifampicin(R),ethambutanol(E)and streptomycin(S)in sputum and lavage samples of 356 hospitalized TB patients were collected in The 3rd People's Hospital of Kunming were detected simultaneously by two testing methods.The results were compared and analyzed by correctionχ^(2) test.Results Single drug resistance,multi-drug resistance and MDR were detected by the two testing methods.On the 99%confidence interval,the adjusted chi-square values are all P<0.01.Conclusion The two methods have differences in the detection of three kinds of resistance.The multidrug resistance PCR-reverse dot blot hybridization method of Mycobacterium tuberculosis drug sensitivity test is better than BD960 detection.The drug sensitivity of single drug resistance and multi-drug resistance BD960 is better than PCR-reverse dot hybridization.
作者
李晓琴
周敏
王霖
曾海燕
骆鹏举
林丽佳
LI Xiao-qin;ZHOU Min;WANG Lin;ZENG Hai-yan;LUO Peng-ju;LIN Li-jia(Dept.of Laboratory,The 3rd People’s Hospital of Kunming,Kunming Yunnan 650301,China;Dept.of Tuberculosis,The 3rd People’s Hospital of Kunming,Kunming Yunnan 650301,China)
出处
《昆明医科大学学报》
CAS
2020年第12期138-141,共4页
Journal of Kunming Medical University
基金
昆明市卫计委医药卫生科技计划基金资助项目(2018-03-02-001)。
关键词
结核分枝杆菌
耐药
基因检测
BD960药敏
Mycobacterium tuberculosis
Drug resistance
Gene detection
BD960 sensitivity
