摘要
目的探讨微小RNA(miR-766-3p)对肺癌细胞增殖、迁移、侵袭的影响及其作用机制。方法采用qRT-PCR与免疫组化分别检测肺癌组织及癌旁组织中miR-766-3p、KIFC1的表达。体外培养肺癌细胞A549,利用脂质体试剂Lipofectamine2000将miR-766-3p mimics、si-KIFC1转染至A549细胞,MTT检测细胞活力;Transwell小室实验检测细胞迁移及侵袭能力;双荧光素酶报告实验验证miR-766-3p与KIFC1的靶向关系。结果 miR-766-3p在肺癌组织中的表达水平显著低于癌旁正常组织,差异有统计学意义(P<0.05),KIFC1的表达水平显著升高,差异有统计学意义(P<0.05)。miR-766-3p过表达与抑制KIFC1的表达后,细胞活力显著降低,迁移与侵袭细胞数显著减少,差异有统计学意义(P<0.05)。结论 miR-766-3p可通过降低肺癌细胞中KIFC1的蛋白水平从而抑制细胞增殖、迁移及侵袭能力。
Objective To investigate the effect of microRNA(miR⁃766⁃3p)on proliferation,mi⁃gration and invasion of lung cancer cells and its mechanism.Methods Real⁃time quantitative polymerase chain reaction(qRT⁃PCR)and Western blot were used to detect the expression of miR⁃766⁃3p and kinesin 1(KIFC1)in lung cancer tissues and adjacent tissues.The lung cancer cell A549 was cultured in vitro,and miR⁃766⁃3p mimics,KIFC1 small interfering RNA(si⁃KIFC1)and their respective negative controls were transfect⁃ed into A549 cells respectively using liposome reagent Lipofectamine 2000.MTT was used to detect cells vitali⁃ty.The Transwell chamber assay detects cell migration and invasion.The dual luciferase reporter assay validated the targeting relationship between miR⁃766⁃3p and KIFC1.Results The expression level of miR⁃766⁃3p in lung cancer tissues was significantly lower than that in adjacent normal tissues(P<0.05),and the expression lev⁃el of KIFC1 was significantly increased(P<0.05).After miR⁃766⁃3p overexpression and inhibition of KIFC1 ex⁃pression,compared with the negative control group,the cell viability was significantly decreased,the number of migration and invasion cells was significantly decreased(P<0.05).Conclusion miR⁃766⁃3p inhibits cell proliferation,migration and invasion by reducing the protein level of KIFC1 in lung cancer cells.
作者
郭喜喜
王振华
胡红军
张立国
GUO Xixi;WANG Zhenhua;HU Hongjun;ZHANG Liguo(Department of Thoracic Oncology,Xinxiang Central Hospital,Xinxiang,Henan,China,453000)
出处
《分子诊断与治疗杂志》
2020年第12期1621-1625,1630,共6页
Journal of Molecular Diagnostics and Therapy
基金
2015年国家自然科学基金项目(81503414)。
