摘要
目的:探讨转化生长因子β 1(TGF-β 1)与二甲基精氨酸二甲胺水解酶2(DDAH2)在早期肺腺癌不同阶段病变中的表达情况及其意义,为肺腺癌精准病理诊断提供依据。 方法:选取山西白求恩医院2012年1月至2019年6月62例早期肺腺癌患者手术切除标本,大部分标本包含1种以上病理类型。按照病理类型分为3组:非典型腺瘤样增生(AAH)18例、原位腺癌(AIS)60例、微浸润腺癌(MIA)、以附壁生长方式为主的浸润性腺癌(LPA)中的浸润性癌(CA)成分55例,选取各样本癌旁正常肺组织作为对照。采用免疫组织化学法检测TGF-β 1、DDAH2蛋白在各病理类型病变组织和对照组织中的表达情况,分析二者的相关性。 结果:TGF-β 1、DDAH2蛋白均在正常肺组织上皮细胞中表达,阳性率分别为46.8%(29/62)、98.4%(61/62);在AAH、AIS及CA组织上皮细胞中,TGF-β 1、DDAH2蛋白阳性率均依次升高,其中TGF-β 1蛋白阳性率分别为16.7%(3/18)、31.7%(19/60)、70.9%(39/55),DDAH2蛋白阳性率分别为66.6%(12/18)、81.7%(49/60)、90.1%(49/55),各病理类型间二者阳性率差异均有统计学意义(均 P<0.01)。TGF-β 1、DDAH2蛋白在正常肺组织、AAH、AIS及CA组织间质成纤维细胞中阳性率均呈依次升高的趋势,其中TGF-β 1蛋白阳性率分别为11.3%(7/62)、61.1%(11/18)、72.3%(44/60)、83.6%(46/55),DDAH2蛋白阳性率分别为0(0/62)、22.2%(4/18)、65.0%(39/60)、98.2%(54/55),各病理类型间二者阳性率差异均有统计学意义(均 P<0.01)。肺腺癌病变组织中,上皮细胞TGF-β 1与间质成纤维细胞DDAH2蛋白表达呈正相关( r=0.221, P=0.011)。 结论:TGF-β 1、DDAH2蛋白在早期肺腺癌各病理类型病变组织中的表达趋势可能与病变程度相关,二者联合检测有望成为辅助诊断早期肺腺癌不同病理类型的生物学标志物。
Objective To investigate the expressions and significances of transforming growth factorβ1(TGF-β1)and dimethylarginine dimethylaminohydrolase 2(DDAH2)in different stages of early-stage lung adenocarcinoma,and to provide basis for accurate pathological diagnosis of lung adenocarcinoma.Methods Sixty-two surgical specimens from patients with early-stage lung adenocarcinoma in Shanxi Bethune Hospital from January 2012 to June 2019 were selected.Most of the specimens contained more than one pathological type.According to the pathological types,they were divided into three groups:18 cases of atypical adenomatous hyperplasia(AAH),60 cases of adenocarcinoma in situ(AIS),and 55 cases of invasive carcinoma(CA)component in minimally invasive adenocarcinoma(MIA)and lepidic predominant adenocarcinoma(LPA),and the wall attached growth patterns were selected as control group.Immunohistochemistry was used to detect the expressions of TGF-β1 and DDAH2 proteins in different pathological types of lesions and control tissues,and the correlation among them was analyzed.Results TGF-β1 and DDAH2 proteins were expressed in normal lung tissue epithelial cells,and the positive rates were high[46.8%(29/62)and 98.4%(61/62)].The positive rates of TGF-β1 and DDAH2 proteins in epithelial cells of AAH,AIS and CA increased gradually,the positive rate of TGF-β1 protein was 16.7%(3/18),31.7%(19/60)and 70.9%(39/55),the positive rate of DDAH2 protein was 66.6%(12/18),81.7%(49/60)and 90.1%(49/55),and the differences of positive rates among different pathological types were statistically significant(all P<0.01).The positive rates of TGF-β1 and DDAH2 protein in interstitial fibroblasts of normal lung tissue,AAH,AIS and CA increased gradually,the positive rate of TGF-β1 protein was 11.3%(7/62),61.1%(11/18),72.3%(44/60),and 83.6%(46/55),the positive rate of DDAH2 protein was 0(0/62),22.2%(4/18),65.0%(39/60),and 98.2%(54/55),and the differences of positive rates among different pathological types were statistically significant(all P<0.01).In lung
作者
侯丽娟
李丽
王琳
张振华
胡丽娜
司彩风
刘小红
Hou Lijuan;Li Li;Wang Lin;Zhang Zhenhua;Hu Lina;Si Caifeng;Liu Xiaohong(Graduate School,Shanxi Medical University,Taiyuan 030001,China;Department of Pathology,Shanxi Bethune Hospital,Taiyuan 030032,China)
出处
《肿瘤研究与临床》
CAS
2020年第11期760-765,共6页
Cancer Research and Clinic
基金
山西省卫生健康委员会科研课题(2018012)。
