摘要
本研究旨在分析不同精粗比日粮对努比亚山羊瘤胃菌群结构的影响。将36只健康、体重相近的努比亚母羊随机分成3组,每组12只羊。每组试验羊分别饲喂精粗比为40∶60(L)、50∶50(M)和60∶40(H)的全混合日粮,试验为期70 d。结果表明:1)3组共产生1901个OTU(操作分类单元,operational taxonomic units),其中共有OTU为1348个,占总OTU数量的70.91%。L组的OTU数量显著高于H组(P<0.05),OTU数量随日粮精粗比升高呈下降趋势。2)Chao指数和Ace指数均随日粮精粗比升高呈下降趋势,其中L组的Ace指数显著高于H组(P<0.05),而各试验组的香农指数和辛普森指数均无显著差异。3)通过聚类树分析,L组和H组的瘤胃菌群结构较近。4)在门水平上,L组的迷踪菌门(Elusimicrobia)、黏胶球型菌门(Lentisphaerae)、TM7、蓝藻菌门(Cyanophyta)、螺旋体门(Spirochaetes)相对丰度均显著高于H组(P<0.05),而广古菌门(Euryarchaeota)相对丰度则相反;L组的变形菌门(Proteobacteria)和疣微菌门(Verrucomicrobia)相对丰度显著高于M组(P<0.05)。5)在属水平上,L组的vadinCA11和Sphaerochaeta相对丰度显著高于H组(P<0.05),而新月形单胞菌属(Selenomonas)和甲烷微球菌属(Methanimicrococcus)相对丰度则相反。6)在种水平上,M组的脱硫弧菌属(Desulfovibrio)D168、白色瘤胃球菌(Ruminococcus albus)、反刍兽新月单胞菌(Selenomonas ruminantium)和栖瘤胃普雷沃菌(Prevotella ruminicola)相对丰度显著高于L组(P<0.01或P<0.05),而黄色瘤胃球菌(Ruminococcus flavefaciens)和产琥珀酸丝状杆菌(Fibrobacter succinogenes)相对丰度则相反。综上,日粮不同精粗比日粮能够影响山羊瘤胃细菌菌群结构,进而影响营养物质的消化吸收。
The purpose of this study was to analyze the effects of diets with different concentration-roughage ratios on the microbial community structure of Nubian goat rumen.Thirty-six healthy Nubian ewes with similar body weights were randomly divided into three groups,with 12 goats per group.Goats in each group were fed total mixed rations with concentration-roughage ratios of 40∶60(L),50∶50(M),or 60∶40(H).The experimental period was 70 days.The results were as follows:1)A total of 1901 OTUs were generated,of which 1348 OTUs were shared by the three groups,accounting for 70.91%of the total.The number of OTUs was significantly higher in group L than in group H(P<0.05).The number of OTUs decreased with the increasing dietary concentration-roughage ratios.2)The Chao and Ace indexes declined with increasing dietary concentration-roughage ratios.The Ace index was significantly higher in group L than in group H(P<0.05).The Shannon and Simpson indexes showed no significant differences between the groups.3)According to cluster analysis,the structure of the rumen microbial community was relatively close in groups L and H.4)At the phylum level,the relative abundances of Elusimicrobia,Lentisphaerae,TM7,Cyanobacteria,and Spirochaetes were significantly higher in group L than group H(P<0.05),but the relative abundance of Euryarchaeota had the opposite result.The relative abundances of Proteobacteria and Verrucomicrobia were significantly higher in group L than group M(P<0.05).5)At the genus level,the relative abundances of vadinCA11 and Sphaerochaeta were significantly higher in group L than group H(P<0.05),but the relative abundances of Selenomonas and Methanimicrococcus had the opposite result.6)At the species level,the relative abundances of Desulfovibrio D168,Ruminococcus albus,Selenomonas ruminantium,and Prevotella ruminicola were significantly higher in group M than group H(P<0.01 or P<0.05),but the relative abundances of Ruminococcus flavefaciens and Fibrobacter succinogenes had the opposite result.In conclusion,the die
作者
霍俊宏
方绍培
吴平山
钟小军
占今舜
HUO Junhong;FANG Shaopei;WU Pingshan;ZHONG Xiaojun;ZHAN Jinshun(Institute of Animal Husbandry and Veterinary,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,Jiangxi,China;Animal Husbandry and Aquatic Products Bureau of Xiushui County,Xiushui 332400,Jiangxi,China)
出处
《草业科学》
CAS
CSCD
2020年第12期2558-2566,共9页
Pratacultural Science
基金
江西省现代农业产业技术体系建设专项(JXARS-13)
江西现代农业科研协同创新专项(JXXTCX201702-04)
江西省草地畜牧业重大技术协同推广计划项目(2018-2020)。
关键词
营养水平
粗饲料
精饲料
全混合日粮
纤维降解菌
蛋白降解菌
瘤胃发酵
nutritional level
roughage
concentrate
total mixed ratios
fibrolytic bacteria
proteolytic bacteria
rumen fermentation
