摘要
微流控芯片是一项以芯片为基础,结合化学、物理学、生物学等多学科形成的技术平台,能够在一个芯片上实现样品制备、反应、分离、检测等流程,具有高通量、高效率和易操作的特点。随着材料科学和纳米技术的发展,该项技术得到迅速发展并应用于化学分析、微生物检测等,尤其是在微生物检测和分析方面发挥着重要作用,并逐步产生了商品化的微流控芯片。微流控芯片因具有小型化、节约试剂、速度快、集成化程度高等优点,适用于处理突发公共卫生事件,但目前在动物疫病检测中的应用还很少见。本文对微流控芯片的技术原理、结构及其在微生物检测中的应用进行综述,以期为动物疫病的高通量检测提供借鉴。
Microfluidic chip is a kind of technology platform based on a chip,which combines with several disciplines,such as chemistry,physics,biology and others,and could complete the processes of preparation,reaction,separation and detection of samples on a chip due to its characteristics of high throughput,high efficiency and easy operation.With the development of material science and nanotechnology,the technology has been rapidly developed and applied to chemical analysis,microbial detection and so on,in which,it has been playing an important role in microbial detection and analysis,and gradually leads to the commercial microfluidic chip that is advantaged with miniaturization,reagent saving,fast and high integration,and appropriate for dealing with public health emergencies,but is still seldom used for animal disease detection at present.In the paper,the technical principles and structure of microfluidic chip as well as its application in microbial detection were reviewed,with a view to providing some references for high-throughput detection of animal diseases.
作者
王楷宬
孙瑞妮
姜楠
姜利建
庄青叶
李阳
王素春
张富友
赵成龙
潘子豪
孙福亮
刘华雷
Wang Kaicheng;Sun Ruini;Jiang Nan;Jiang Lijian;Zhuang Qingye;Li Yang;Wang Suchun;Zhang Fuyou;Zhao Chenglong;Pan Zihao;Sun Fuliang;Liu Hualei(China Animal Health and Epidemiology Center,Qingdao,Shandong 266032,China;Qingdao Yebio Biological Engineering Co.,Ltd.,Qingdao,Shandong 266114,China;Nanjing Agricultural University,Nanjing,Jiangsu 210095,China;Yanbian University,Yanji,Jilin 133000,China;Shandong Agricultural University,Tai'an,Shangdong 271018,China)
出处
《中国动物检疫》
CAS
2021年第1期87-93,共7页
China Animal Health Inspection
基金
国家重点研发计划项目(2017YFC1200500)。
关键词
微流控芯片
原理
结构
微生物
应用
microfluidic chip
principle
structure
microorganism
application
