摘要
目的:研究萝卜硫素(SF)是否可通过上调转录因子NF-E2相关因子2(Nrf2)起到抗氧化和抗炎作用降低急性肺损伤(acute lung injury,ALI)。方法:选取健康小鼠32只随机分为急性肺损伤组(ALI组)、对照组(对照组)、SF组和SF溶剂组,每组各8只;对照组腹腔注射10mg/kg生理盐水;SF组腹腔注射20mg/kg(SF),SF溶剂组腹腔注射20mg/kg(SF载体)溶剂,2次/d,连续注射3d,第4d时给予ALI组和SF组、SF溶剂组注射脂多糖(LPS)10mg/kg,并制备内毒素诱发ALI模型。LPS或生理盐水注射6h后取下腔静脉血,并使用ELISA检测血清IL-6和TNF-α水平含量;肺组织病理结果通过光镜观察后对肺损伤进行评分;小鼠肺组织内Nrf2核蛋白表达使用Western blotting检测,并同时计算肺干重/湿重比;应用比色法检测肺组织匀浆中SOD、MPO和iNOS含量。结果:ALI组与SF溶剂组肺损伤评分、iNOS、MPO、IL-6和TNF-α水平明显较对照组升高,Nrf2蛋白表达上调(P<0.05),且肺干重/湿重比和SOD较对照组降低(P<0.05);ALI组与SF溶剂组比较上述各项指标差异无统计学意义(P>0.05);SF组IL-6、TNF-α、肺损伤评分和iNOS含量明显较ALI组降低,但肺干重/湿重比和SOD含量升高,Nrf2蛋白表达上调(P<0.05)。结论:经SF通过诱导Nrf2蛋白表达可减轻LPS诱导急性肺损伤的严重程度,还可降低IL-6、TNF-α和MPO水平,证实Nrf2转录因子对ALI可起到有效保护作用。
Objective:To study whether sulforaphane(SF)can reduce the acute lung injury(ALI)by up-regulating the transcription factor NF-E2 related factor 2(Nrf2)to play an antioxidant and anti-inflammatory role.Methods:Thirty-two healthy mice were randomly divided into acute lung injury group(ALI group),control group(control group),SF group,and SF solvent group,8 mice in each group.The control group was injected intraperitoneally with 10 mg/kg saline.SF group was intraperitoneally injected with 20 mg/kg(SF),SF solvent group was intraperitoneally injected with 20 mg/kg(SF carrier)solvent,2 twice a day,continuous injection was performed for 3 days,and ALI group,SF group,and SF solvent group were injected with lipopolysaccharide at the 4th day.(LPS)10 mg/kg,and an endotoxin-induced ALI model was prepared.Six hours after injection of LPS or normal saline,vena cava blood was removed and serum IL-6 and TNF-ɑ levels were measured by ELISA.Pulmonary tissue pathological results were scored for lung injury after observation with light microscope.Nrf2 nucleus in mouse lung tissue Protein expression was measured by Western blotting,and lung dry weight/wet weight ratio was calculated simultaneously.Colorimetric method was used to detect SOD,MPO and iNOS content in lung tissue homogenate.Results:The lung injury score,iNOS,MPO,IL-6,and TNF-ɑ levels in the ALI group and the SF solvent group were significantly higher than those in the control group,and the expression of Nrf2 protein was up-regulated(P<0.05).SOD was lower than that in the control group(P<0.05).There was no significant difference in the above indicators between the ALI group and the SF solvent group(P>0.05).The IL-6,TNF-ɑ,lung injury score,and iNOS content in the SF group were significant.Compared with the ALI group,the lung dry weight/wet weight ratio and SOD content increased,and the expression of Nrf2 protein was up-regulated(P<0.05).Conclusion:SF can reduce the severity of LPS-induced acute lung injury by inducing the expression of Nrf2 protein through SF,and it can al
作者
刘振峰
刘代顺
刘建英
周国旗
李云飞
曾静新
LIU Zhenfeng;LIU Daishun;LIU Jianying(The Third Affiliated Hospital of Zunyi Medical University,Guizhou Zunyi 563000,China)
出处
《河北医学》
CAS
2020年第12期1961-1965,共5页
Hebei Medicine
基金
贵州省卫生健康委科学技术基金项目,(编号:Gzwjki2016-1-019)
贵州省遵义市“15851人才精英工程”科研资助项目。
