摘要
以芦丁、尿素和三氯化铁为原料,水热法合成了铁掺杂碳点(Fe-CDs),并对制备的Fe-CDs进行了透射电子显微镜、X-射线光电子能谱和傅里叶红外光谱的表征。Fe-CDs表面富含-COOH、-OH和-NH2等含氧官能团,粒径主要分布在0.5~5 nm。Fe-CDs可催化H2O2氧化3,3',5,5'-四甲基联苯胺,并在652 nm处出现较强的吸收峰。而谷胱甘肽(GSH)能还原ox-TMB使体系吸收峰强度降低。基于上述原理,建立了以Fe-CDs为过氧化物模拟酶的GSH测定方法。GSH浓度与体系吸收峰强度的变化值(ΔA)在2~30μmol·L^-1浓度范围内呈良好的线性关系,检出限为0.8μmol·L^-1。将该方法用于测定实际样品谷胱甘肽片的含量,加标回收率在98%~103%,RSD为1.4%~2.2%。结果表明,该方法能够用于实际样品谷胱甘肽片中GSH的含量测定。
Iron-doped carbon dots(Fe-CDs)was synthesized from rutin,urea and ferric chloride by hydrothermal method and was characterized by using TEM images,XPS,FTIR spectroscopy.There are rich-COOH,-OH and-NH2 groups on the surface of Fe-CDs,particle size mainly distribution in 0.5~5 nm.Fe-CDs can catalyse H2 O2 oxidation TMB reaction and there emerged a strong absorbance at 652 nm.However,GSH can reduce ox-TMB then reduce the absorbance intensity of system.Based on the above prin-ciples,a method with Fe-CDs as peroxidase mimetic enzyme for GSH detection was established.There was a good linear relationship between GSH and changes intensity of absorbance peak(ΔA)in the range from 2 to 30μmol·L^-1,the detection of limited(3σ/k)was 0.8μmol·L^-1.The content of GSH in sample was investigated with this method,the adding standard recovery was 98%~103%and RSD was 1.4%~2.2%.The results showed that this method is simple and intuitive,and can determine the content of GSH in actual samples.
作者
孟铁宏
杨湘怡
姜艳萍
陶晨
罗亚男
任竹君
李咏梅
胡先运
李春荣
MENG Tie-hong;YANG Xiang-yi;JIANG Yan-ping;TAO Chen;LUO Ya-nan;REN Zhu-jun;LI Yong-mei;HU Xian-yun;LI Chun-rong(Qiannan Medical College for Nationalities,Duyun 558000,China;The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550002,China)
出处
《化学研究与应用》
CAS
CSCD
北大核心
2020年第12期2133-2138,共6页
Chemical Research and Application
基金
国家自然科学基金项目(81860317)资助
贵州省自然科学基金项目(黔科合基础[2020]1Y051,黔科合基础[2019]1301号,黔科合支撑[2019]2821号)资助
黔南州科技计划项目(黔南州科合社字[2017]19号)资助
黔南民族医学高等专科学校基金项目(QNYZ201902,QNYZ201801,QNYZ201807,QNYZ201706)资助。
关键词
铁掺杂碳点
过氧化物模拟酶
谷胱甘肽
iron-doped carbon dots
peroxidase mimetic enzyme
glutathione
