摘要
【目的】研究单质硫对β-HgS在胃肠道条件下汞溶出的影响及机制.【方法】模拟佐太中β-HgS和单质硫的组成,采用体外溶出试验方法,测定β-HgS、β-HgS与单质硫混合物在含或不含L-半胱氨酸的模拟胃、肠液及不同pH条件下溶出的汞浓度,测定β-HgS在不同浓度Na2S,Na2SO3或Na2S2O3的含L-半胱氨酸的模拟肠液中溶出的汞浓度.【结果】单质硫对β-HgS中汞溶出的影响及其与pH、L-半胱氨酸关系的试验表明,单质硫可抑制β-HgS在不含L-半胱氨酸的模拟肠液和含L-半胱氨酸的模拟胃、肠液中汞的溶出;该抑制作用与溶液pH条件有关,与溶液中是否含有L-半胱氨酸关系较小.硫离子形态对β-HgS在含L-半胱氨酸的模拟肠液中汞溶出影响的试验表明,Na2S和Na2S2O3分别在5~50、1000 mg/L下抑制β-HgS中汞溶出的作用与单质硫的抑制作用相似.【结论】单质硫是造成β-HgS在不含L-半胱氨酸的模拟肠液和含L-半胱氨酸的模拟胃、肠液中溶出的汞浓度显著降低的主要因素;单质硫主要通过生成S2-来抑制β-HgS在含L-半胱氨酸的模拟肠液中的汞溶出.
【Objective】To investigate the effect and mechanism of elemental sulfur on Hg dissolution ofβ-HgS under the condition of gastrointestinal tract.【Method】The composition ofβ-HgS and elemental sulfur in Zuotai was simulated and the in-vitro-dissolution test was used to measure the concentration of dissolved Hg fromβ-HgS or the mixture ofβ-HgS and elemental sulfur in simulated gastric and intestinal fluids under different pH values with or without L-cysteine.And the concentration of dissolved Hg fromβ-HgS was measured in L-cysteine-containing simulated intestinal fluid at different concentrations of Na2S,Na2SO3 or Na2S2O3.【Result】The effect of elemental sulfur on Hg dissolution ofβ-HgS and its relationship with pH and L-cysteine showed that elemental sulfur inhibited Hg dissolution ofβ-HgS in L-cysteine-free simulated intestinal fluid and L-cysteine-containing simulated gastric juice and intestinal fluids.And this inhibition effect was related to the pH value of solutions,but less related to L-cysteine.The effect of sulfide ion morphology on Hg dissolution ofβ-HgS in L-cysteine-containing simulated intestinal fluid showed that Na2S and Na2S2O3 respectively inhibited the Hg dissolution at 5~50 and 1000 mg/L,which were similar to elemental sulfur.【Conclusion】Elemental sulfur was the main factor significantly reducing the concentration of dissolved Hg ofβ-HgS in L-cysteine-free simulated intestinal fluid and L-cysteine-containing simulated gastric juice and intestinal fluids.Elemental sulfur inhibited the Hg dissolution ofβ-HgS in L-cysteine-containing simulated intestinal fluid mainly by producing S2-.
作者
刘志滔
张明
耿卢婧
杜玉枝
魏立新
尚靖
LIU Zhi-tao;ZHANG Ming;GENG Lu-jing;DU Yu-zhi;WEI Li-xin;SHANG Jing(Qinghai Provincial Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China;Key Laboratory of Tibetan Medicine Research,Chinese Academy of Sciences,Xining 810008,China;University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2020年第6期19-25,33,共8页
Journal of Gansu Agricultural University
基金
青海省重点实验室专项(2017-ZJ-Y08)
中国科学院“西部之光”计划项目(Y529021211)
中国科学院国际合作局对外合作重点项目(153631KYSB20160004)。
关键词
佐太
单质硫
β-HgS
模拟胃肠道
汞溶出
Zuotai
elemental sulfur
β-HgS
simulated gastrointestinal tract
Hg dissolution
