摘要
选择上海闪晶分子生物科技有限公司、南京诺维赞生物科技有限公司、北京蓝谱生物科技有限公司的Bst酶和LAMP反应缓冲液,进一步对猪圆环病毒2型(PCV2)环介导等温扩增(LAMP)试剂盒的原材料进行了研究。结果表明:南京诺维赞生物科技有限公司的试剂较好,该厂家的原材料可实现试剂盒检测性能的最优化。该组合灵敏度高,阳性参考品稀释度为10-11(5个病毒∕μL)可进行反应,且特异性较好。本研究可为后续药证申报奠定基础,同时为其他相关LAMP诊断试剂盒的药证申报提供参考。
The raw materials of loop-mediated isothermal amplification(LAMP)kit for porcine circovirus type 2(PCV2)were further studied by using Bst enzyme and LAMP reaction buffer from Shanghai ShineGene Molecular Biotechnology Co.,Ltd.,Nanjing Vazyme Biotechnology Co.,Ltd.and Beijing Lanpu Biotechnology Co.,Ltd..The results showed that the reagent of Nanjing Vazyme Biotechnology Co.,Ltd.was better,and the raw materials of the manufacturer could optimize the detection performance of the kit.The sensitivity of the combination was high,when the dilution of positive reference material was 10-11(5 viruses∕μL),the reaction could be carried out,and the reaction was specific.This study could lay a foundation for the later declaration of drug certificate,and provide reference for other related LAMP diagnostic kits.
作者
张琪
张皖静
张晓霞
赵悦琪
吴文静
袁文天
许燕
赵凯
ZHANG Qi;ZHANG Wanjing;ZHANG Xiaoxia;ZHAO Yueqi;WU Wenjing;YUAN Wentian;XU Yan;ZHAO Kai(College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;Shanghai Bio-Full Biotechnology Company Limited,Shanghai 201106,China;Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China)
出处
《上海农业学报》
2020年第6期66-71,共6页
Acta Agriculturae Shanghai
基金
上海市科学技术委员会2017年科技创新行动计划(17391901800)。
