摘要
目的分析深圳市男男性行为者(MSM)中唾液和血液HIV快速检测试剂结果,探讨唾液快速检测法在MSM人群HIV筛查的作用。方法同时采集深圳市393名MSM人群的唾液和血液样本,使用HIV免疫印迹试剂盒作为参考试剂,比较唾液抗体检测试剂盒(胶体金法)和人类免疫缺陷病毒(HIV)1+2型抗体检测试剂盒(胶体硒法)两种快速检测试剂结果的符合性。结果在393份MSM有效样本中,唾液检测试剂的真阳性为120例,假阳性为0例,真阴性为272例,假阴性为1例。血液快速法真阳性为121例,假阳性为3例,真阴性为269例,假阴性为0例。以HIV免疫印迹试剂盒检测结果为金标准,唾液检测试剂的灵敏度为99.2%,特异度为100.0%,假阳性率为0.0%,假阴性率为0.8%,准确度为99.8%。血液检测试剂的灵敏度为100.0%,特异度为98.9%,假阳性率为1.1%,假阴性率为0.0%,准确度为99.2%。Kappa检验分析显示唾液、血液快速检测法与HIV诊断结果存在一致性,Kappa值分别为0.994和0.982;McNe-mar配对卡方检验分析唾液和血液快速法的检测结果发现二者的差异无统计学意义(P=0.125)。结论唾液与血液的筛查结果具有较好一致性,基于唾液检测安全无创、快速,该方法值得在高危人群HIV抗体自我筛查中推广使用。
Objective To compare the results of HIV rapid detection reagent in saliva and blood in MSM in Shenzhen,and we clarify the role of saliva rapid detection in HIV screening in this population.MethodsThe exudate in saliva and blood samples of the same subjects in 393 MSM in Shenzhen were collected,and the HIV immunoblotting kit was used as the reference reagent to confirm the consistency of salivary antibody detection kit(colloidal gold method)and human immunodeficiency virus(HIV)type 1+2 antibody detection kit(colloidal selenium method).ResultsTotally 393 MSM were recruited.The true positive,false positive,true negative,false negative cases for saliva test reagents were 120,0,272,1,respectively.Those for blood test reagents were 121,3,269,0,respectively.The detection results of the HIV western blot kit were taken as the gold standard to compare and analyze the detection results of saliva and blood detection reagent.The sensitivity,specificity,false-positive rate,false-negative rate and accuracy of exudate in saliva test reagents were 99.2%,100.0%,0.0%,0.8%and 99.8%,respectively.Those of blood test reagents were 100.0%,98.9%,1.10%,0.0%and 99.2%,respectively.Kappa test analysis showed that the results of rapid saliva and blood test were consistent with HIV diagnosis,and the Kappa values were 0.994 and 0.982,respectively.McNemar paired chi-square test was used to analyze the results of saliva and blood rapid test,and the difference was not statistically significant(P=0.125).ConclusionSaliva and blood screening results have a good consistency,exudate in saliva detection is noninvasive and fast,this method is worth promoting for HIV self-screening in high risk population.
作者
甘永霞
李浩
杨峥嵘
赵锦
石向东
高石兰
GAN Yongxia;LI Hao;YANG Zhengrong;ZHAO Jin;SHI Xiangdong;GAO Shilan(Shenzhen Center for Disease Control and Prevention,Shenzhen,Guangdong 518055,China)
出处
《中国热带医学》
CAS
2020年第12期1172-1174,共3页
China Tropical Medicine
基金
广东省医学科学技术研究基金项目(No.A2019474)
深圳市引进高层次医学团队项目“三名工程”(No.SZSM201811071)
深圳市卫生系统科研项目(No.SZGW2018003)。
