摘要
目的:本研究采用基因重组的慢病毒液灌注糖尿病膀胱病变(DCP)豚鼠膀胱的方式,观察其对膀胱逼尿肌收缩功能的影响。方法:建立DCP豚鼠模型,将DCP模型随机分为实验组、阳性对照组和空白对照组,实验组灌注SCL基因重组慢病毒液;阳性对照组灌注未携带SCL基因的慢病毒+PBS液;空白对照组只灌注PBS液;检测灌注后对各组豚鼠逼尿肌收缩功能的影响。结果:实验组中灌注后随时间的延长,逼尿肌收缩功能有所恢复,空白组和阳性对照组灌注后,逼尿肌的收缩功能均无明显改善。结论:经尿道灌注SCL基因(人类干细胞白血病基因)重组慢病毒转染DCP膀胱后,豚鼠的膀胱逼尿肌收缩功能较前有所改善,提示该方法对DCP具有疗效,为DCP行基因治疗提供了依据。
Objective:To investigate the effect of recombinant Lentivirus on the contractile function of bladder detrusor in guinea pigs with diabetic bladder disease(DCP).Methods:DCP guinea pig model was established and randomly divided into experimental group,positive control group and blank control group.The experimental group was perfused with recombinant lentivirus of SCL gene;the positive control group was perfused with lentivirus+PBS solution without SCL gene;the blank control group was perfused with PBS solution only;the effect of perfusion on detrusor contraction function was detected.Results:in the experimental group,the detrusor systolic function recovered with the prolongation of time,but there was no significant improvement in the contraction function of detrusor in the blank group and positive control group after perfusion.Conclusion:Transurethral instillation of recombinant lentivirus of human stem cell leukemia gene(SCL gene)into DCP bladder can improve the contractile function of bladder detrusor in guinea pigs,which indicates that this method is effective for DCP and provides the basis for gene therapy of DCP.
作者
张艺星
王勤章
ZHANG Yi-xing;WANG Qin-zhang(Shihezi University School of Medicine,Xinjiang Shihezi,832002;Department of Urology,The First Affiliated Hospital of Shihezi University School of Medicine,Xinjiang Shihezi,832008)
出处
《农垦医学》
2020年第3期229-233,共5页
Journal of Nongken Medicine
