摘要
为了解广西地区血清4型禽腺病毒(FAdV-4)的基因特征,本研究对实验室前期分离的一株FAdV-4(命名为GX2019-010)进行了全基因组测序,对所获核苷酸序列进行同源性比对和遗传进化分析,并进一步分析了主要结构蛋白Hexon、Penton和Fiber的氨基酸序列。结果显示,GX2019-010全长43719 bp,GC含量为54.9%,主要包括43个潜在蛋白质编码区域。核苷酸序列同源性比对发现,GX2019-010与致病株MX-SHP95的同源性为95.8%,与非致病株ON1和KR5同源性分别为95.0%和95.2%。遗传进化分析显示,GX2019-010与中国FAdV-4近几年的流行毒株在同一分支,而与国外FAdV-4毒株不在同一分支,说明FAdV-4存在遗传差异,主要的差异存在于基因组的右端,国内分离株均出现1966 bp的缺失,其中包括ORF19、ORF48和ORF27基因的缺失,说明中国流行的FAdV-4具有地域性。对主要结构蛋白Hexon、Penton和Fiber的氨基酸序列分析发现,3个主要结构蛋白均有氨基酸位点的突变,其中Fiber-2的突变位点最多。本研究丰富了广西地区FAdV-4的基因库,为今后心包积液-肝炎综合征(HHS)的防控及流行病学调查研究提供了参考依据。
To understand the genetic characteristics of fowl adenovirus serotype 4(FAdV-4)in Guangxi region,the whole-genome sequencing were performed on a FAdV-4 strain GX2019-010 isolated from Guangxi in this study.Genome-wide nucleotide sequences were analyzed for homology alignment and genetic evolution,and the amino acid sequences of the major structural proteins Hexon,Penton and Fiber were further analyzed.The results showed that GX2019-010 had a full length of 43719 bp and GC content of 54.9%,mainly including 43 potential protein coding regions.Genome-wide nucleotide homology comparisons revealed that GX2019-010 was 95.8%homologous to the pathogenic strain MX-SHP95,and 95.0%and 95.2%homologous to non-pathogenic strains ON1 and KR5,respectively.Genetic evolutionary analysis showed that GX2019-010 was in the same branch as the popular strains of FAdV-4 in China in recent years,but not in the same branch as the foreign strains of FAdV-4.This results indicated that there were genetic differences in FAdV-4.The main differences was at the right end of the genome and all domestic strains isolated in China were 1966 bp missing including ORF19,ORF48 and ORF27 genes.It showed that FAdV-4 popular in China was territorial.Analysis of the amino acid sequences of the major structural proteins of Hexon,Penton and Fiber revealed that all of the three major structural proteins had mutations in amino acid sites,of which Fiber-2 had the most mutations.This study enriched the gene pool of FAdV-4 in Guangxi region and laid the foundation for future research on the prevention and control of HHS and epidemiological investigation.
作者
栾勇娇
谢芝勋
王盛
罗思思
张蕾
谢丽基
谢志勤
邓显文
张民秀
张艳芳
曾婷婷
范晴
LUAN Yongjiao;XIE Zhixun;WANG Sheng;LUO Sisi;ZHANG Lei;XIE Liji;XIE Zhiqin;DENG Xianwen;ZHANG Minxiu;ZHANG Yanfang;ZENG Tingting;FAN Qing(College of Animal Science and Technology,Guangxi University,Nanning 530004,China;Guangxi Key Laboratory of Veterinary Biotechnology,Guangxi Veterinary Research Institute,Nanning 530001,China)
出处
《中国畜牧兽医》
CAS
北大核心
2020年第12期3793-3804,共12页
China Animal Husbandry & Veterinary Medicine
基金
广西科技基地和人才专项(桂科AD17195083)
广西科技重大专项(桂科AA17204057)
广西“八桂学者”专项(2019-79)
国家“万人计划”领军人才专项(W02060083)。
