摘要
【目的】明确绿盲蝽(Apolygus lucorum Meyer-Dür)对果园常用杀虫剂的敏感性差异,并建立其对这些杀虫剂的敏感基线和抗药性检测的诊断剂量,为生产上推断绿盲蝽对不同药剂的抗性发生状况以及合理用药提供理论依据。【方法】采用玻璃管药膜法测定9种杀虫剂对绿盲蝽室内敏感品系三龄若虫的毒力水平,并用死亡概率值法计算得到LC50值和LC99值。以LC50值作为敏感基线数据,以两倍LC99值作为诊断剂量数据。【结果】供试的9种杀虫剂对绿盲蝽的毒力从高到低为:联苯菊酯(LC50=0.79 mg·L^-1)、毒死蜱(3.96 mg·L^-1)、高效氯氟氰菊酯(6.25 mg·L^-1)、噻虫嗪(10.47 mg·L^-1)、甲氰菊酯(10.91 mg·L^-1)、马拉硫磷(29.42 mg·L^-1)、啶虫脒(36.26 mg·L^-1)、吡虫啉(78.29 mg·L^-1)和氟啶虫胺腈(160.89 mg·L^-1)。并得出各杀虫剂相应的敏感基线和抗性诊断剂量。【结论】测定了9种果园常用杀虫剂对实验室敏感品系绿盲蝽的毒力水平,并提供了敏感基线和诊断剂量,为测定和评估田间绿盲蝽抗性及其发展趋势提供了理论依据。
【Objective】The mired bug,Apolygus lucorum Meyer-Dür,has gradually become an important fruit tree pest in Yellow River region of China in recently year.For apple tree,both nymphs and adults suck plant juices through their needle-like mouthparts,and their feeding can induce the stunting of plant young tissue and the abscission of flower buds even young fruits,finally leading to serious yield and quality losses.Currently,management of A.lucorum relies exclusively on chemical insecticides,including pyrethroids,organophosphates,and neonicotinoids.Although some of these insecticides have been used for a long time and insect susceptibilities are declining in cotton filed,they are still effective in the field for the control of A.lucorum.However,continues and dominant use of chemical sprays will facilitated pesticide resistance in this pest.Therefore,it is urgent and essential to estimate insecticide resistance in the mired bug in orchards for proper choice of insecticides.Developing tools for insecticide resistance detection and monitoring is a key component of resistance management.To make clear the sensitivity difference of A.lucorum to frequently-used pesticides in orchard and establish the susceptible baseline date and the diagnostic doses of insecticides for detecting resistance,a series of toxicity of nine insecticides to the susceptible strain of A.lucorum were conducted in this study.【Methods】The susceptibilities of third instar nymph of A.lucorum laboratory strain were tested by the glass-vial method bioassay.Glass-vial bioassays were conducted in 5-mL glass vials with an internal surface area of 8 cm2.The stock solutions(2000 g·L^-1)of all insecticides tested were prepared in acetone.Then,5 concentrations of the working solutions were prepared by diluting the stock solution in 0.01%Triton X-100 in acetone.50 mL working solution was added to the vials that were then manually rolled horizontally until the acetone had completely evaporated.Control vials were treated with acetone and 0.01% Triton X-100.I
作者
赵钧
涂洪涛
张金勇
陈汉杰
ZHAO Jun;TU Hongtao;ZHANG Jinyong;CHEN hanjie(Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,Henan,China;Institute of Tobacco Research,Henan Academy of Agricultural Sciences/Key Laboratory for Green Preservation&Control of Tobacco Diseases and Pests in Huanghuai Growing Area,Zhengzhou 450002,Henan,China)
出处
《果树学报》
CAS
CSCD
北大核心
2020年第12期1974-1979,共6页
Journal of Fruit Science
基金
国家重点研发计划(2016YFD0201100)
中国农业科学院科技创新工程(CAAS-ASTIP)
国家现代农业(苹果)产业技术体系专项基金(CARS-27)。
关键词
果园
绿盲蝽
杀虫剂
玻管药膜法
抗药性
敏感基线
Orchard
Apolygus lucorum
Insecticide
Glass-vial method bioassay
Pesticide resistance
Susceptible baseline data
