摘要
目的从分子水平研究皮肤鳞状细胞癌(CSCC)血清蛋白组学的变化,为该病的发生、发展机制研究及分子靶向药物的研发提供理论依据。方法基于高分辨质谱的全扫描数据非依赖采集(Data Independent Acquisition,DIA)定量蛋白质组学技术,比较CSCC患者与正常人血清中蛋白种类和浓度的差异。而后根据这些差异蛋白进行生物信息分析:GO功能及富集分析、Pathway代谢通路富集和蛋白-蛋白相互作用网络(PPI)分析。结果在待测血清样品中共检测到411个蛋白。通过对比6个CSCC和6个正常人血清样品的蛋白图谱特征,发现共有28个蛋白具有显著性差异。GO富集分析结果表明biological process(BP)、cellular component(CC)和molecular function(MF)三个本体中分别有533个、62个和57个显著性差异的条目(GO terms)。KEGG pathway分析提示这些差异蛋白显著地富集在pantothenate and coA biosynthesis、MAPK signaling pathway、propanoate metabolism、pyruvate metabolism和cysteine and methionine metabolism五条代谢通路上。进一步的PPI分析表明10条代谢通路和8个蛋白相互关联,具体包括癌症相关的代谢途径glycolysis/gluconeogenesis、MAPK signaling pathway等,以及与癌症发生密切关联的的蛋白CTSB、LDHB等。结论一些潜在的生物标志物可能用于早期诊断CSCC,同时MAPK信号通路在该病的发生发展过程中具有十分重要的作用。
Objective In order to provide novel insights into the pathophysiological mechanism and therapy options for CSCC.Methods Data-independent mode(DIA)-based SWATH-MS was used for 12 pooled samples from the CSCC patients’(n=6)and controls’sera(n=6).Further bioinformatics analyzes were conducted based on deferentially expressed proteins including GO enrichment analysis,pathway enrichment analysis,and protein-protein interaction network analysis.Results A total of 411 proteins were identified in the detected samples,28 of which were determined to be significantly different(p value<0.05)between CSCC patients(n=6)and controls(n=6).In GO enrichment analysis based on the deferentially expressed proteins,533 biological process terms,62 molecular function terms,and 57 cellular component terms were significantly enriched.While in the pathway enrichment analysis,pantothenate&coA biosynthesis,MAPK signaling pathway,propanoate metabolism,propanoate metabolism,and pyruvate metabolism were significantly enriched.Further protein-protein interaction network analysis showed 10 pathways and 8 proteins were significantly correlated including several cancer related pathways/proteins such as glycolysis/gluconeogenesis,MAPK signaling pathway,CTSB,and LDHB.Conclusion This paper not only provide insight into the potential biomarkers of CSCC for diagnostic and prognostic purposes,but suggest the important role of MAPK signaling pathway involved in the physiopathology of this syndrome.
作者
陈雯
刘智文
游小龙
袁分钱
张丽
乐飞
高源
饶军
CHEN Wen;LIU Zhiwen;YOU Xiaolong(Jiangxi Cancer Hospital,Nanchang,330029)
出处
《实用癌症杂志》
2020年第12期1928-1931,共4页
The Practical Journal of Cancer
基金
江西省科技厅重点研发计划项目(编号:20181BBG78041)
江西省卫健委课题项目(编号:20203577,20203192)。
