摘要
采用水提醇沉法从流苏子中提取多糖,Sevage法除蛋白,利用DEAE-52纤维和Sephadex G-100凝胶柱色谱进行分离纯化,得到多糖CRp-1,采用化学和仪器分析方法研究了多糖的总糖和蛋白质含量、单糖组成、官能团特征、空间构像等理化性质,并通过体外实验研究CRp-1对α-葡萄糖苷酶的抑制活性。结果显示,从流苏子中分离的多糖CRp-1为白色粉末,溶于水,不溶于有机溶剂;总糖含量为97.12%,蛋白质含量为1.65%,不含单糖和多酚,不属于淀粉多糖;主要由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖组成,其摩尔比为1∶5.381∶1.146∶10.804∶6.953∶11.908,相对分子质量分别为223200 g/mol;紫外和红外光谱显示具有多糖的特征吸收峰,且含有吡喃糖苷。CRp-1具有三股螺旋结构,且表面为三维多孔结构,并带有一定的圆形颗粒。体外α-葡萄糖苷酶抑制活性实验表明,CRp-1具有一定的α-葡萄糖苷酶抑制活性,具有浓度依赖性。
Polysaccharide CRp-1 was extracted from Chionanthus retusus Lindl.et Paxt.seed by water extraction and alcohol precipitation,protein was removed by Sevage method,and purified by DEAE-52 fiber and Sephadex G-100gel column chromatographys.The physicochemical properties,such as total sugar and protein contents,monosaccharide composition,functional group characteristics,spatial conformation were studied by chemical and instrumental analysis,and the inhibitory activity of CRp-1 onα-glucosidase was studied in vitro.The results showed that the polysaccharide CRp-1 that isolated from C.retusus seed was white powder,soluble in water and insoluble in organic solvents,the total sugar and protein contents were 97.12%and 1.65%.CRp-1 did not contain monosaccharides and polyphenols,and also not belong to starch polysaccharides.CRp-1 was mainly composed of rhamnose,arabinose,xylose,mannose,glucose and galactose,with a molar ratio of 1∶5.381,1.146,10.804:6.953:11.908,and the molecular weight was 223200 g/mol.UV and IR spectra showed that there were characteristic absorption of polysaccharides and contained furanoside.CRp-1 had a triple helix structure,and the surface was a three-dimensional porous structure,with some round particles.The experiment ofα-glucosidase inhibitory activity in vitro showed that CRp-1 had certainα-glucosidase inhibitory activity in a concentration-dependent manner.
作者
马天晓
尹震花
张翠利
刘会利
张金香
郭玮玮
陈林
MA Tian-xiao;YIN Zhen-hua;ZHANG Cui-li;LIU Hui-li;ZHANG Jin-xiang;GUO Wei-wei;CHEN Lin(Zhengzhou Key Laboratory of Synthetic Biology of Natural Products,Henan Joint International Research Laboratory of Drug Discovery of Small Molecules,Huanghe Science and Technology College,Zhengzhou 450063,China;Department of Pharmacy,Affiliated Hospital of Huanghe Science and Technology College,Zhengzhou 450063,China)
出处
《食品工业科技》
CAS
北大核心
2020年第24期64-68,80,共6页
Science and Technology of Food Industry
基金
国家林业和草原局科技发展中心生物安全和遗传资源管理项目(KJZXSA2019044)
河南省科技攻关项目(182102110143)
郑州市基础研究及应用基础研究专项资金项目(ZZSZX202003)。
关键词
流苏子
多糖
理化性质
α-葡萄糖苷酶抑制活性
Chionanthus retusus Lindl.et Paxt.seed
polysaccharide
physicochemical property
α-glucosidase inhibitory activity
