摘要
目的建立RP-HPLC法同时测定回生口服液中山柰酚、阿魏酸和迷迭香酸3种活性成分的含量。方法采用Shim-pack CLC-ODS C_(18)色谱柱分离;检测波长:342 nm;柱温:32℃;进样体积:20μL;流动相:乙腈(A)-1 mL·L^(-1)甲酸(B);梯度洗脱,洗脱程序为:0~5 min,11%A;5~18 min,11%A^35%A;18~26 min,35%A^69%A;26~28 min,69%A^11%A;29~35 min,11%A。外标法定量检测。结果 3种待测组分质量浓度均在0.1~20.0μg·mL^(-1)范围内线性关系良好;重复性和精密度RSD值均小于5.0%(n=6);平均回收率分别为101.12%,98.26%和98.21%;供试品溶液在24 h内稳定性良好。结论此方法简单、快速,结果准确,重复性、精密度和稳定性均较好,可用于回生口服液中中成药产品的质量控制。
Objective To develop a reverse phase high-performance liquid chromatography(RP-HPLC)method for simultaneously determining the contents of kaempferol,ferulic acid and rosmarinic acid in Huisheng Oral Liquid.Methods Shim-pack CLC-ODS C 18 column was selected for separation,and detection wavelength was set at 342 nm.The column temperature was 32℃,and the injection volume was 20μL.The mobile phase was acetonitrile(A)and 1 mL·L-1 formic acid(B).Gradient elution procedure was as follows:0-5 min,11%A;5-18 min,11%A-35%A;18-26 min,35%A-69%A;26-28 min,69%A-11%A;29-35 min,11%A.External standard method was used for quantitative detection.Results The mass concentrations of the 3 components showed good linear relationships in the range of 0.1 to 20.0μg·mL-1.The RSD of repeatability and precision were all below 5.0%(n=6).The average recoveries of the 3 active components were 101.12%,98.26%and 98.21%,respectively.The sample solution was stable within 24 h.Conclusion This method is simple,fast,accurate,reproducible,precise and stable,and can be used for the quality control of Chinese patent medicine Huisheng Oral Liquid.
作者
曲芳菲
董林
QU Fangfei;DONG Lin(Yantai Affiliated Hospital of Binzhou Medical College,Yantai 264100,China)
出处
《西北药学杂志》
CAS
2020年第6期836-838,共3页
Northwest Pharmaceutical Journal
