摘要
采用石墨脉冲电解法制备了碳量子点溶胶,利用透射电子显微镜、X射线衍射仪、傅里叶变换红外光谱和荧光光谱等手段对碳量子点的形状、尺寸、结构和性能进行了表征。碳量子点为近似球形,平均粒径为6.93 nm,表面含有多种富氧官能团并具有荧光特性。体外细胞毒性试验表明:当碳量子点浓度高于一定值时,溶胶能诱导人体肝癌细胞凋亡,对其具有显著的抑制作用。对碳量子点诱导凋亡后的人体肝癌细胞进行了透射电子显微镜观察,结果显示其对线粒体产生了损伤。在相同的浓度范围内,碳量子点溶胶对人体淋巴细胞几乎没有产生影响。碳量子点溶胶具有选择性诱导人体肝癌细胞凋亡的作用,对于研究新型无毒副作用的抗癌药物具有重要意义。
The carbon quantum dots(CQDs)colloid solution was prepared by electrolyzing graphite with pulse current.The shape,size,structure and performance of the carbon quantum dots were characterized by transmission electron microscopy,X-ray diffractometer,Fourier transform infrared spectroscopy and fluorescence spectroscopy,etc.The CQDs were quasi-spherical particles with an average particle size of 6.93 nm,and the surface contained a variety of oxygen-rich functional groups and showed fluorescence characteristics.The cytotoxicity test showed that the colloid solution could induce the apoptosis of human hepatoma cells(HepG2 cells),and thus could effectively inhibit the proliferation of the cells,when the concentration of CQDs colloid solution was above a certain value.Transmission electron microscopy images of HepG2 cells after apoptosis induced by CQDs showed that their mitochondria were damaged.There was almost no effects on human lymphocytes in the same CQDs concentration range.The CQDs colloid solution has the effect of selectively inducing apoptosis of HepG2 cells,which is of great significance for the research of new anti-cancer drugs without side effects.
作者
张涛
郭一民
李卓扬
席果果
左磊
曹永平
王雅泓
ZHANG Tao;GUO Yimin;LI Zhuoyang;XI Guoguo;ZUO Lei;GAO Yongping;WANG Yahong(School of Materials Science and Engineering,Zhengzhou University,Zhengzhou 450001,China;School of Materials Science and Engineering,Beihang University,Beijing 100191,China;Orthopaedics,Peking University First Hospital,Beijing 100034,China;Harbin Chengcheng Institute for Life and Material,Harbin 150500,China)
出处
《郑州大学学报（工学版）》
CAS
北大核心
2020年第5期1-7,共7页
Journal of Zhengzhou University（Engineering Science）
基金
国家自然科学基金资助项目(51971006,51571005)。
关键词
碳量子点
溶胶
癌细胞
人体淋巴细胞
凋亡
carbon quantum dots
colloid solution
cancer cells
human lymphocytes
apoptosis
