摘要
目的分析Polo样蛋白激酶4(PLK4)在结直肠癌(CRC)及癌旁组织中的表达水平,探讨其与临床病理参数的相关性及临床预后评估价值。方法选取石蜡包埋CRC组织和癌旁组织158对,通过免疫组织化学技术检测PLK4在癌组织及配对癌旁组织中的表达。结果在158例CRC患者中PLK4蛋白在癌组织的阳性率为70.3%(111/158)在癌旁组织的阳性率为41.1%(65/158),癌组织中的表达率高于癌旁组织(P<0.05);复发/死亡患者癌组织PLK4的表达水平高于非复发/生存患者(均P<0.05);PLK4低表达、Ⅱ期CRC患者的DFS和OS长于PLK4高表达、Ⅲ期患者(均P<0.05);PLK4和TNM分期均是CRC患者独立的预后因素(均P<0.05)。结论PLK4基因在CRC中的表达显著高于癌旁组织,是CRC患者独立的预后因素,可能成为CRC潜在的治疗靶点。
Objective To analyze the expression level of PLK4 in colorectal cancer(CRC)and adjacent tissues,and to explore its correlation with clinicopathological parameters and clinical prognostic evaluation value.Methods158 pairs of paraffin-embedded CRC tissues and adjacent tissues were selected.Immunohistochemistry was used to detect the expression of PLK4 in cancerous tissues and paired adjacent tissues.Results Among 158 CRC patients,the positive rate of PLK4 protein in cancer tissues was 70.3%(111/158),and the positive rate in adjacent tissues was41.1%(65/158).The expressionof PLK4 protein in cancer tissues was significantly higher than that in adjacent cancers(<0.05).The expression level of PLK4 in cancer tissues of relapsed/dead patients was significantly higher than that of non-relapsed/surviving patients(<0.05).Both DFS and OS of CRC patients with low PLK4 expression as well as stage II were significantly longer than those of patients with high PLK4 expression and stage III(all<0.05).PLK4 and TNM stages were independent prognostic factors for CRC patients(all<0.05).Conclusions The expression of PLK4 gene in CRC tissues is significantly higher than that in adjacent tissues.PLK4 acts as an independent prognostic factor for CRC patients and may become a potential clinical therapeutic target.
作者
沈晓涵
陈佳意
李征
周新成
SHEN Xiaohan;CHEN Jiayi;LI Zheng;ZHOU Xincheng(Ningbo Diagnostic Pathology Center Ningbo 315000,Zhejiang,China)
出处
《现代实用医学》
2020年第11期1311-1313,1356,I0001,共5页
Modern Practical Medicine
基金
浙江省医药卫生科研基金项目(2017KY607)
宁波市自然科学基金(2016A610186)。
关键词
结直肠肿瘤
癌
PLK4
蛋白表达
预后
Colorectal neoplasm
Cancer
PLK4
Protein expression
Prognosis
