摘要
目的探讨不同破膜剂对于流式细胞术检测小肠中3型固有淋巴样细胞(ILC3)频率的影响。方法取小鼠小肠组织,经胶原酶消化后获得ILC3,比较BD公司破膜剂(BD组)、eBioscience公司破膜剂(EB组)、联科生物公司破膜剂(MS组)对小肠中ILC3检测频率的影响。结果在其他条件相同情况下,EB组和BD组检测到小肠内ILC3的频率高于MS组(P<0.05),但小肠ILC3的检出频率在BD组和EB组之间的差异无统计学意义(P>0.05)。结论不同类型破膜剂对小肠ILC3检出频率有影响,实验时应根据细胞类型选择合适的破膜剂。
Objective To explore the effect of different fixation/permeabilization reagents on the detection of innate lymphoid cells 3(ILC3)frequency in the intestine by flow cytometry.Methods The intestine tissues of mice were obtained,and digested it with collagenase to get ILC3.The effects of different fixation/permeabilization reagents from Becton Dickinson and Company(BD group),eBioscience Company(EB group)and Multi Sciences(Lianke)Biotechnology Corporate Limited(MS group)on the detection results of small intestinal ILC3 were compared.Results Under the same other conditions,the frequency of ILC3 detection in small intestine in EB group and BD group was higher than that in MS group(P<0.05),but there was no significant difference between BD group and EB group(P>0.05).Conclusion Different fixation/permeabilization reagents may affect the detection frequency of ILC3 in the small intestine,and the appropriate fixation/permeabilization reagents should be selected according to the cell type during the experiment.
作者
曹敏
张晓敏
王培昌
刘静
CAO Min;ZHANG Xiaomin;WANG Peichang;LIU Jing(Department of Clinical Laboratory,Xuanwu Hospital,Capital Medical University,Beijing 100053,China)
出处
《国际检验医学杂志》
CAS
2020年第23期2835-2838,共4页
International Journal of Laboratory Medicine
基金
国家自然科学基金项目(81472007)
北京市医管局人才培养计划“登峰”项目(DFL20180803)。
关键词
流式细胞术
破膜剂
3型固有淋巴样细胞
flow cytometry
fixation/permeabilization reagents
innate lymphoid cells 3
