摘要
目的:观察丹参素对银屑病小鼠皮损中Yes相关蛋白(YAP)表达影响。方法:用外用咪喹莫特的方式建立银屑病鼠模型,予以不同浓度丹参素(20、40、80mg·kg^-1·d^-1)进行灌胃处理,7d后观察鼠皮损的大体及组织病理情况,免疫组化及Western blot检测鼠皮损中YAP的表达。结果:模型组小鼠皮肤增厚,出现红斑鳞屑,予以丹参素灌胃后可见皮损逐渐变薄、鳞屑减少。模型组小鼠YAP的表达增加(P<0.01),丹参素灌胃后其表达下降,并且这一下降趋势随着丹参素浓度的增加而增大。结论:丹参素可能通过影响YAP的表达进而抑制了银屑病皮损进展。
Objective:To observe the effects of tanshinol on Yes-associated protein in lesion of psoriatic mice.Methods:The mouse model of psoriasis was established by external imiquimod.Tanshinol(20,40,80 mg·kg^-1·d^-1)was administered by gavage.After 7 days,the general and histopathological changes of the lesions were observed.The expression of YAP in the lesions was detected by immunohistochemistry and Western blot.Results:In imiquimod group,the skin became thicker and erythematous scales appeared.After administration of tanshinol,the skin lesion became thinner and the scales decreased.After administration of imiquimod,the expression of YAP increased(P<0.01),but decreased after the use of tanshinol,and the decreasing trend increased with the increase of tanshinol concentration.Conclusion:Tanshinol may inhibit the progress of psoriasis by affecting the expression of YAP.
作者
贾金靖
莫秀梅
晏烽根
郑焱
李红毅
陈达灿
JIA Jin-jing;MO Xiu-mei;YAN Feng-gen;ZHENG Yan;LI Hong-yi;CHEN Da-can(State Key Laboratory of Dampness Syndrome of Chinese Medicine,No.2 Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China;Department of Dermatology,No.2 Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China;Guangdong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chinese Diseases,Guangzhou 510120,China;Department of Dermatology,the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2020年第11期5783-5786,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金青年科学基金项目(No.81803136)
陕西省自然科学基础研究计划一般项目(青年)(No.2018JQ8067)
广东省中医药防治难治性慢病重点实验室(No.2018B030322012)。
