摘要
目的:建立二黄益肾汤的质量控制标准。方法:采用薄层色谱法(TLC)对二黄益肾汤中的丹参和黄芪进行定性鉴别;反相-高效液相色谱法(RP-HPLC)对大黄中的大黄酸、大黄素、大黄酚进行含量测定。结果:TLC能定性检出丹参和黄芪,斑点清晰,阴性无干扰;大黄酸、大黄素、大黄酚分别在41.625~666.0μg·mL^-1(r=1.0000)、2.663~42.6μg·mL^-1(r=0.9999)、6.94~111.0μg·mL^-1(r=0.9999)具有良好的线性关系,平均回收率分别为97.45%(RSD=0.39%)、98.63%(RSD=1.18%)、98.15%(RSD=0.89%)(n=6)。结论:所建方法专属性强,能有效检测二黄益肾汤的质量,可作为二黄益肾汤的质量控制标准。
Objective To establish the quality standard of Erhuang Yishen Decoction.Methods Thin layer chromatography(TLC)was used for the qualitative identification of Salvia miltiorrhiza and Astragalus membranaceus.Reversed phase high performance liquid chromatography(RP-HPLC)was used for the quantitative determination of rhein,emodin and chrysophanol in Rheum officinale.Results TLC could accurately detected the presence of Salvia miltiorrhiza and Astragalus membranaceus,the spots were clear and the blank test showed no interference.The concentration of rhein,emodin and chrysophanol showed a good linearity in the ranges of 41.625~666.0μg·mL^-1(r=1.0000),2.663~42.6μg·mL^-1(r=0.9999)and 6.94~111.0μg·mL^-1(r=0.9999),respectively.Additionally,the average recovery was 97.45%(RSD=0.39%),98.63%(RSD=1.18%)and 98.15%(RSD=0.89%),respectively.Conclusion The specificity of method is very good as well,and can effectively detect the quality of Erhuang Yishen decoction,and can be used as quality control detection standard.
作者
李冬冬
田雪芬
王静
周玮
张丹丹
LI Dongdong;TIAN Xuefen;WANG Jing;ZHOU Wei;ZHANG Dandan(Jining Hospital of Traditional Chinese Medicine,Jining 272037,China)
出处
《中国民族民间医药》
2020年第21期50-54,共5页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
济宁市科技助推新旧动能转换计划项目(2017SMNS005)。
