摘要
目的:探讨畅脉乐胶囊对颈动脉粥样硬化大鼠Wnt信号通路的影响。方法:将30只大鼠随机分为对照组、模型组、治疗组,每组10只。除对照组外,以高脂饮食为诱变剂建立大鼠颈动脉粥样硬化模型。治疗组按5 mg/(kg·d)的剂量喂食畅脉乐胶囊,各组给予相应饲料喂养,16周后采血并处死。检测大鼠血脂中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)水平;蛋白质印迹法检测大鼠颈动脉硬化斑块处Wnt1、β-连环蛋白(β-catenin)、dvl-1蛋白表达情况。结果:与对照组比较,模型组大鼠TC、TG、LDL-C水平及颈动脉硬化斑块处Wnt1、β-catenin、dvl-1蛋白表达水平升高(P<0.05)。与模型组比较,治疗组大鼠TC、TG、LDL-C水平及颈动脉硬化斑块处Wnt1、β-catenin、dvl-1蛋白表达水平降低(P<0.05)。结论:畅脉乐胶囊可通过抑制大鼠Wnt信号通路中Wnt1、β-catenin、dvl-1的表达减轻颈动脉粥样硬化程度。
Objective: To investigate the effect of Changmaile capsules on Wnt signaling pathway in rats with carotid atherosclerosis. Methods:A total of 30 rats were randomly divided into the control group,the model group and the treatment group, with 10 rats in each group. Except for those in the control group, the rat models of carotid atherosclerosis were established by using high-fat diet as mutagen. The treatment group was fed Changmaile capsules at a dose of 5 mg/(kg·d),and each group was given corresponding feed. After 16 weeks,they were taken blood sampling and then were sacrificed.The levels of total cholesterol(TC), triglyceride(TG) and low-density lipoprotein cholesterol(LDL-C) in blood lipid were detected;the protein expressions of Wnt1,β-catenin and dvl-1 in carotid atherosclerosis plaques in rats were detected by Western blotting. Results : Compared with those in the control group, the levels of TC, TG, LDL-C and the protein expressions of Wnt1, β-catenin and dvl-1 in carotid atherosclerosis plaques in the model group were all increased(P<0.05). Compared with those in the model group,the levels of TC,TG,LDL-C and the protein expressions of Wnt1,β-catenin and dvl-1 in carotid atherosclerosis plaques in the treatment group were all decreased(P<0.05). Conclusion:Changmaile capsules can reduce the degree of carotid atherosclerosis by inhibiting the expressions of Wnt1,β-catenin and dvl-1 in Wnt signaling pathway in rats.
作者
谢胜伟
陈美华
XIE Shengwei;CHEN Meihua
出处
《新中医》
CAS
2020年第21期10-12,共3页
New Chinese Medicine
基金
2018年福建省中青年教师教育科研项目(JT180235)。
