摘要
目的:探讨可溶性CD40配体(sCD40L)对人非霍奇金淋巴瘤细胞增殖、凋亡及细胞周期的影响及其可能机制。方法:用不同浓度的sCD40L处理非霍奇金淋巴瘤CA46细胞和Raji细胞48 h,用CCK-8检测细胞增殖;流式细胞术检测细胞凋亡和周期;通过蛋白免疫印迹检测PTEN、BCL-2和BAX的表达。结果:与对照组比较,4和8μg/ml的sCD40L均能显著抑制Raji细胞和CA46细胞的增殖(P<0.05)。流式细胞术检测凋亡和细胞周期结果显示,4μg/ml的sCD40L能显著促进CA46和Raji细胞的凋亡和减少S期细胞的比例(P<0.05);蛋白免疫印迹结果显示,sCD40L能显著促进PTEN和BAX的表达,抑制BCL-2的表达(P<0.05)。结论:sCD40L抑制淋巴瘤细胞的增殖并促进其凋亡可能是通过调控PTEN信号通路实现。
Objective:To explore the effect of soluble CD40 ligand(sCD40L)on the proliferation,apoptosis,and cell cycle of human non-Hodgkin lymphoma(NHL)cells,and analyze its possible mechanism.Methods:NHL CA46 cell and Raji cell were treated with different concentrations of sCD40L for 48 h,CCK-8 was used to detect the effect of sCD40L on cell proliferation in vitro,flow cytometry on apoptosis and cycle of NHL cells,and Western blot on the expression of PTEN,BCL-2,and BAX in NHL cells.Results:Compared with the control group,4 and 8μg/ml sCD40L could significantly inhibit the proliferation of lymphoma Raji cell and CA46 cell(P<0.05).The test results of flow cytometry showed that 4μg/ml sCD40L could significantly promote the apoptosis of CA46 and Raji cells,and significantly inhibit the S phase proportions(P<0.05).Western blot results showed that sCD40L could promote the expression of PTEN and BAX,while inhibit the expression of BCL-2(P<0.05).Conclusion:sCD40L can promote the apoptosis and inhibit the proliferation of NHL cells through the PTEN signaling pathway.
作者
封忠昕
王季石
陈琦
FENG Zhong-Xin;WANG Ji-Shi;CHEN Qi(Department of Hematology of Affiliated Hospital of Zunyi Medical University,Zunyi 563003,Guizhou Province,China;Guizhou Medical University Graduate School,Guiyang 550004,Guizhou Province,China;Department of Hematology of Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou Province,China)
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2020年第6期1933-1938,共6页
Journal of Experimental Hematology
基金
贵州省科技厅联合基金项目(黔科合LH字[2017]7092号)。
