摘要
本试验从苹果(Malus pumila Mill)转录组数据库中挖掘SSR标记,发现其SSR标记类型共有六种,分别为:单核苷酸、二核苷酸、三核苷酸、四核苷酸、五核苷酸和六核苷酸,其中频率较高的重复类型为单核苷酸、二核苷酸和三核苷酸。从41 890条苹果无冗余转录组序列中共搜索到了1 803条至少含有1个SSR的序列,占转录组的4.30%。高频重复类型为单核苷酸、二核苷酸和三核苷酸,其中数量最多的是A/T,其次是AG/CT和GAG/CTC,共占SSRs的98.23%。该试验共设计了20对引物,分别对20份苹果材料进行PCR扩增。结果显示:20对引物均能扩增出条带,平均扩增条数为2.7。并对供试的20份苹果品种进行了聚类分析,结果与形态学及生物学分类基本一致,证明品种间亲缘关系的远近具有一定的规律,栽培品种与砧木品种的种间亲缘关系较远,而栽培种和砧木品种的种内亲缘关系较近。另外,本研究为杂交育种挑选合适的亲本,杂交育种以及培育作物新品种提供了一定的理论基础。
Six types of SSR markers were found in Malus pumila Mill transcriptome database: Mononucleotides,dinucleotides, trinucleotides, tetranucleotides, pentonucleotides, and hexonucleotides, The highest frequency of repeating types: mononucleotides, dinucleotides, and trinucleotides. From 41 890 apple non-redundant transcriptome sequences, a total of 1 803 sequences containing at least one SSR were searched, accounting for 4.30% of the transcriptome. The high frequency repeats were mononucleotides, dinucleotides and trinucleotides, accounting for98.23% of the total SSRs. The most common primitive types were A/T, followed by AG/CT and GAG/CTC. Twenty pairs of polymorphic primers were designed and synthesized, and 20 apple materials were amplified by PCR. The results showed that all the 20 pairs of primers could amplify obvious bands, among which 11 pairs of primers amplified 30 polymorphic bands, average polymorphic bands were 2.7 SSR marker technology was used for cluster analysis of apple varieties tested. The results were basically consistent with morphological and biological classification, which indicated that there was a certain pattern between cultivars and rootstock cultivars. The interspecific relationship between cultivated cultivars and rootstock cultivars was pretty far, while the intraspecific relationship between cultivated cultivars and rootstock cultivars was relatively close. This study provides a theoretical basis for selecting suitable parents, for hybrid breeding and cultivating new crop varieties.
作者
苏艳丽
毛娟
马宗桓
何红红
卢世雄
王萍萍
陈佰鸿
Su Yanli;Mao Juan;Ma Zonghuan;He Honghong;Lu Shixiong;Wang Pingping;Chen Baihong(College of Horticulture,Gansu Apriculural Univesity,Luanzhou,730070)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第22期7450-7457,共8页
Molecular Plant Breeding
基金
国家自然基金(31860530)
甘肃省高等学校产业支撑引导项目(2019C-11)
甘肃省科技重大专项(18ZD2-NA006)共同资助。
