摘要
为探究双粒基因tg1对稻米品质的影响,本研究以‘浙粳22’的双粒突变体‘浙粳22-tg1’为供体,将tg1基因导入大粒品种‘SLG’,比较了‘浙粳22’、‘浙粳22-tg1’、‘SLG’和‘SLG-tg1’在灌浆速率、稻米品质指标和淀粉颗粒形态上的不同,分析双粒基因tg1对稻米品质的影响,发现新品系‘SLG-tg1’灌浆速率高,在稻米品质包括外观品质、加工品质以及蒸煮食味和营养品质上都有显著改善。淀粉颗粒的电镜扫描观察表明,与‘SLG’相比,‘SLG-tg1’淀粉颗粒结构更加紧密,为优质米类型的多面体形。因此,本研究发现转育tg1基因可改善稻米品质,为选育优质水稻新品种提供参考。
To explore the effect of twin-grain1(tg1) gene on rice quality, a japonica twin-grain mutant ’Zhejing 22-tg1’ was used as a donor to transfer the tg1 gene to a large-grain variety ’SLG’. Grain filling rate(GFR), rice quality and starch granule morphology were compared among ’Zhejing 22’, ’Zhejing 22-tg1’, ’SLG’ and ’SLG-tg1’. The effect of the tg1 on grain quality was analyzed. The new line ’SLG-tg1 ’showed increased GFR and significantly improved grain quality including appearance quality, processing quality, cooking taste and nutritional quality.Scanning electron microscope(SEM) observation of starch granules showed that the starch granule structure of’SLG-tg1’ is much more compact than ’SLG’ with a multi-sided shape, which meant its good grain quality.Therefore, this study demonstrated that introduction of the tg1 gene could improve grain quality in rice and it provided a reference for breeding new high-quality rice varieties.
作者
叶胜海
翟荣荣
叶靖
朱国富
陆艳婷
张小明
Ye Shenghai;Zhai Rongrong;Ye Jing;Zhu Guofu;Lu Yanting;Zhang Xiaoming(Insitute of Crop and Nuclear Technology Utiliztion,Zhejiang Academy of Agricultural Sciences,Hangzhou,310021)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第22期7423-7428,共6页
Molecular Plant Breeding
基金
浙江省农业(粮食)新品种选育重大科技专项(2016C02050-5)
浙江省基础公益研究计划(LGN18C130004,LGN20C130005)共同资助
关键词
水稻
双粒基因
稻米品质
灌浆速率
淀粉颗粒结构
Rice
tg1 gene
Grain quality
Grain filling rate GFR
Starch granule structure
