摘要
目的为研究一株羊种布鲁氏菌粗糙型变异菌株的遗传特征。方法本研究利用布鲁氏菌表型鉴定方法、全基因组测序及比较基因组分析方法。结果分离株与单因子R血清、三胜黄素发生凝集且能够被结晶紫染色,全基因组测序表明,该分离株与羊种2型菌株相似性最高;其基因组由2条染色体组成,大小为3311700 bp,GC含量为57.2%,含有3379个CDS,9个rRNA,55个tRNA和4个ncRNA;存在282个变异,其中243个SNPs,39个InDel;已知的LPS合成基因中,8个存在错义突变。结论基因水平上的突变导致LPS合成受阻,分离株表型由光滑型变为粗糙型。本研究为羊种布鲁氏菌粗糙型变异菌株的研究提供了基础数据,同时也为布鲁氏菌疫苗候选株的筛选提供新思路。
The aim of this study was to analyze the genetic characteristics of the B.melitensis strain with a rough phenotype.In this study,rough phenotype detection methods,whole-genome sequencing and comparative genome analysis methods were used.The results showed that this isolated strain was agglutinated with R monospecific sera and trisavin,and could be stained with crystal violet.The genome sequence of the isolation was similar to B.melitensis bv.2 str.ATCC23457 at the genomic level.The genome of the isolation consisted of two chromosomes,with a size of 3311700 bp and a GC content of 57.2%,including 3379 CDS,9 rRNA,55 tRNA and 4 ncRNA.There are 282 variations,including 243 SNPs and 39 InDel.Among them,eight of the known LPS-related genes had missense variations.In conclusion,because these variations at the gene level hindered LPS synthesis,the phenotype of the isolation changed from smooth to rough.This study provided the basic data for rough B.melitensis,and new insights for the screening of Brucella vaccine strains.
作者
杨晓雯
韩秀瑞
朴东日
赵鸿雁
田国忠
姜海
YANG Xiao-wen;HAN Xiu-rui;PIAO Dong-ri;ZHAO Hong-yan;TIAN Guo-zhong;JIANG Hai(State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Bejing 102206,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2020年第11期894-899,共6页
Chinese Journal of Zoonoses
基金
国家自然科学基金(No.81902105)
侯云德院士科研青年基金项目(No.2019HYDQNJJ08)
艾滋病和病毒性肝炎等重大传染病防治(No.2018ZX10712-001)联合资助。
