摘要
目的探讨不同运动方式对2型糖尿病小鼠血清短链脂肪酸(short-chain fatty acid,SCFA)的影响,明确运动改善2型糖尿病小鼠SCFA的最佳运动方式,为早期糖尿病患者采取预防性干预措施提供理论依据。方法根据不同运动方式,将8周龄雄性db/db 2型糖尿病小鼠48只随机分为有氧运动组、抗阻运动组、联合抗阻-有氧运动(简称联合运动)组及对照组。每组各10只小鼠;另外每组再各增加2只作为替补。各组小鼠喂养方式一致。对照组不作运动干预;有氧运动组进行无负重跑步运动;抗阻运动组进行尾部负重爬梯运动;联合运动组隔日交替进行有氧运动和抗阻运动;于干预前、干预8周后检测小鼠血糖、体重及使用气相色谱法检测小鼠血清SCFA的含量。结果 40只小鼠均顺利完成实验。干预前,各组血糖、体重比较,差异均无统计学意义(P>0.05)。干预8周后,有氧运动组、抗阻运动组、联合运动组的血糖、体重均较对照组下降(P<0.05),联合运动组血糖、体重均低于单一的有氧运动、抗阻运动组(P<0.05)。血清中SCFA的含量有氧运动组、抗阻运动组、联合运动组均高于对照组;联合运动组乙酸、丁酸高于有氧运动组,丙酸、戊酸高于抗阻运动组(P<0.05)。结论不同的运动方式能改善2型糖尿病小鼠血清中SCFA的含量,与有氧运动及抗阻运动相比,联合运动在改善SCFA方面效果更佳。
Objective To explore the effects of different exercise methods on serum short-chain fatty acids(SCFA)in type 2 diabetic mice,determine the best exercise method to improve SCFAs in type 2 diabetic mice,and provide a theoretical basis for the preventive intervention for patients with early diabetes.Methods According to different exercise methods,488-week-old male db/db type 2 diabetic mice were randomly divided into four groups,including aerobic exercise group,resistance exercise group,combined resistance-aerobic exercise(referred to as combined exercise)group,and the control group;with 10 mice in each group and another 2 as the substitutes.The mice were fed in the same manner in each group.The control group did not perform exercise intervention,the aerobic exercise group performed weightless running exercise,the resistance exercise group performed tail weight-bearing ladder exercise,and the combined exercise group alternated aerobic exercise and resistance exercise.Blood glucose and body weight were measured before and 8 weeks after the intervention.The content of serum SCFAs in mice was determined by gas chromatography-mass spectrometry.Results A total of 40 mice completed the experiment successfully.Before the exercise intervention,there was no significant difference in blood glucose or weight among the groups(P>0.05).After 8 weeks of exercise intervention,the blood glucose and weight in each exercise group were significantly lower than those in the control group(P<0.05),and the blood glucose and weight in the combined exercise group were significantly lower than those in the aerobic exercise group and the resistance exercise group(P<0.05).The contents of SCFA were higher in the aerobic exercise group,resistance exercise group,and combined exercise group than those in the control group(P<0.05);the contents of acetic acid and butyric acid in the combined exercise group were better than those in the aerobic exercise group(P<0.05),and the contents of propanoic acid and valeric acid in the combined exercise group we
作者
马翠
黄燕凤
翟露
刘玉花
张秋
黄山
钟鑫
戴霞
MA Cui;HUANG Yanfeng;ZHAI Lu;LIU Yuhua;ZHANG Qiu;HUANG Shan;ZHONG Xin;DAI Xia(Department of Nursing,the First Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530021,P.R.China;Stem Cell Transplantation Division,the First Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530021,P.R.China;Pulmonary and Critical Care Medicine WardⅡ,the First Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530021,P.R.China)
出处
《华西医学》
CAS
2020年第11期1370-1375,共6页
West China Medical Journal
基金
国家自然科学基金(81660147)
广西自然科学基金(2018GXNSFAA050091)
南宁市青秀区科技计划项目(2018027)。
关键词
糖尿病
有氧运动
抗阻运动
短链脂肪酸
Diabetes
Aerobic exercise
Resistance exercise
Short-chain fatty acids
