摘要
通过在发酵培养基中添加C1,2位脱氢酶抑制剂,研究抑制剂对分枝杆菌MS23转化植物甾醇制备9-羟基-雄烯二酮(9-OH-AD)的影响。研究表明:宝丹酮十一烯酸酯为相对效果最好的抑制剂,其最适添加时间及浓度分别为72 h、15 mmol·L^-1。在此条件下添加宝丹酮十一烯酸酯,9-OH-AD积累量可达到170.47 mg·L^-1,比对照组的46.07 mg·L^-1提高了2.7倍。此外,通过对C1,2位脱氢酶活性进行分析,进一步验证抑制剂宝丹酮十一烯酸酯对C1,2位脱氢酶存在抑制作用。最后,在分枝杆菌MS23的5 L发酵罐中添加宝丹酮十一烯酸酯,9-OH-AD于132 h后的浓度可达到138.50 mg·L^-1。
Effects of 3-ketosteroid-1-dehydrogenase inhibitors on the bioconversion of phytosterol to 9-hydroxy-4-androstene-3,17-dione(9-OH-AD)by Mycobacterium sp.MS23 were evaluated in this study.Screening experiments of 3-ketosteroid-1-dehydrogenase inhibitors were performed.The results show that boldenone undecylenate is the most effective inhibitor compared to other experimental inhibitors.Furthermore,the optimal addition time and concentration for boldenone undecylenate are 72 h and 15 mmol,respectively.Under optimized conditions,the 9-OH-AD accumulation reaches 170.47 mg·L^-1 with boldenone undecylenate addition,a 2.7-fold increase over the control.In addition,the enzyme activities of 3-ketosteroid-1-dehydrogenase at different reaction periods were measured.It indicates that the boldenone undecylenate has an inhibitory effect on 3-ketosteroid-1-dehydrogenase.Finally,the experiment of converting phytosterol to 9-OH-AD under the condition of adding boldenone undecylenate was carried out in a 5 L fermenter.The concentration of 9-OH-AD reaches 138.50 mg·L^-1 after 132 h.
作者
张孝峰
薛凯
鞠鑫
李良智
ZHANG Xiaofeng;XUE Kai;JU Xin;LI Liangzhi(School of Chemistry and Life Sciences,SUST,Suzhou 215009,China)
出处
《苏州科技大学学报（自然科学版）》
CAS
2020年第4期45-50,共6页
Journal of Suzhou University of Science and Technology（Natural Science Edition）
基金
国家自然科学基金资助项目(21676173)
苏州市应用基础研究项目(SNG2018046)。
关键词
分枝杆菌
C1
2位脱氢酶
宝丹酮十一烯酸酯
9-羟基-雄烯二酮
Mycobacterium sp.MS23
3-ketosteroid-1-dehydrogenase
boldenone undecylenate
9-hydroxy-4-androstene-3,17-dione
