摘要
以吲哚美辛诱导建立GES-1细胞损伤模型,研究猴头菇菌丝体多糖和子实体多糖对胃黏膜的保护作用。采用水提醇沉提取法优化猴头菇粗多糖初步提纯的工艺,并在细胞水平上探究提纯前、后多糖对胃黏膜保护作用的差异。研究显示,猴头菇菌丝体粗多糖和子实体粗多糖对GES-1细胞都有明显的增殖作用,并能明显提高受损GES-1细胞的存活率。以多糖纯度及其对GES-1细胞存活率的影响为评价指标,得到猴头菇粗多糖提纯的最佳工艺条件:料液比1∶15、提取温度80℃、提取时间2 h。在此条件下,菌丝体多糖纯度由40%增至50%,子实体多糖纯度由35%增至42%,受损GES-1细胞的存活率也明显增强。研究表明猴头菇菌丝体多糖和子实体多糖对胃黏膜具有良好的保护作用。
The GES-1 cell injury model was induced by indomethacin to study the protective effect of polysaccharides and fruiting body polysaccharides of Hericium erinaceus on gastric mucosa.The extraction process of crude polysaccharides from Hericium erinaceus was optimized by water extraction and alcohol precipitation extraction method,and the difference of polysaccharides on gastric mucosa protection before and after purification was explored at the cellular level.Studies have shown that the crude polysaccharides of the mycelium of Hericium erinaceus and the crude polysaccharides of the fruiting bodies have obvious proliferative effects on GES-1 cells,and can significantly improve the survival rate of damaged GES-1 cells.The optimum conditions for the purification of crude polysaccharides from Hericium erinaceus were as follows:the ratio of solid to liquid was 1∶15,the extraction temperature was 80℃,and the extraction time was 2 h.Under these conditions,the purity of mycelium polysaccharide increased from 40%to 50%,the purity of fruiting body polysaccharide increased from 35%to 42%,and the survival rate of damaged GES-1 cells also increased significantly.The results indicated that the mycelium polysaccharides and fruiting body polysaccharides from Hericium erinaceus have good protective effects on gastric mucosa.
作者
袁尔东
黄敏
李良
杨宜婷
任娇艳
Yuan Erdong;Huang Min;Li Liang;Yang Yiting;Ren Jiaoyan(School of Food Science and Engineering,South China University of Technology,Guangzhou 510641;Infinitus(China)Company Ltd,Guangzhou 510665;Sino-Singapore International Joint Research Institute,Guangzhou 510006)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2020年第11期71-78,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
“广东特支计划”科技青年拔尖人才项目(2014TQ01N645)
中央高校基本科研业务费项目(2019KZ01)
高等学校学科创新引智计划项目(B17018)。
关键词
猴头菇
多糖
提纯
GES-1细胞
胃黏膜保护
Hericium erinaceus
polysaccharide
purification
GES-1 cell
gastric mucosal protection
