摘要
为了解天麻苦荞复配液的添加对灰树花深层发酵过程中代谢产物的差异性,采用超高效液相色谱-四级杆串联飞行时间质谱(UPLC-QTOF-MS)技术结合多变量统计分析方法,对发酵7d的灰树花菌丝体细胞代谢物进行分析。主成分(PCA)模型显示添加天麻苦荞复配液的菌丝体细胞与对照组菌丝体细胞相比代谢产物差异明显(P<0.05),通过正交偏最小二乘判别分析(OPLS-DA),以VIP(varible importance in the projection)>1和P<0.05为条件进行筛选和鉴定得到44种差异代谢物,包括糖类6种、氨基酸类13种、维生素类5种、核苷酸类7种、有机酸类10种、脂肪酸类3种。其中,与对照组相比鼠李糖、D-半乳糖、D-甘露醇、果糖-6-磷酸等7种物质含量显著下调,D-木糖醇、异亮氨酸、赖氨酸、泛酸、二十二碳六烯酸、D-葡萄糖醛酸、琥珀酸等37种物质含量显著上调。通过对差异代谢物进行通路分析,得到具有显著影响的代谢通路14条,推测了灰树花胞外多糖合成通路。由此推断天麻苦荞复配液的添加对灰树花胞外多糖的增效作用和提升营养品质的原因,为今后深层次研究外源添加物对灰树花发酵过程的影响提供理论依据。
Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS)combined with multivariate statistical methods was used to analyze the differences of intracellular metabolites of Grifola frondosa mycelium cells in submerged fermentation of 7 days by the addition of compound solution of Gastrodia elata and Fagopyrum tataricum.The results showed that the principal component(PCA)model revealed a significant differences between metabolites of mycelium cells cultured in compound solution and solution of experiment control(P<0.05).Through orthogonal partial least squares discriminant analysis(OPLS-DA),44 differential metabolites were screened and identified under the conditions of VIP(varible importance in the projection)>1 and P<0.05,including 6 sugars,13 amino acids,5 vitamins,7 nucleotides,10 organic acids and 3 fatty acids.The content of 7 substances such as L-rhamnose monohydrate,D-galactose,D-mannitol,fructose-6-phosphate was significantly reduced,the content of 37 substances such as D-xylitol,isoleucine,lysine,pantothenate,dihydroxyacetone,D-glucuronic acid,succinate increased significantly as compared with the experimental control.Pathway analysis of differential metabolites indicated that there were 14 metabolic pathways with significant impact and the syuthetic pathway of Grifola frondosa extracellular polysaccharide was constructed.The results proved the synergistic effect of the addition of Gastrodia elata and Fagopyrum tataricum compound solution on the production of extracellular polysaccharide of Grifola frondosa thereby improving the nutritional quality.Further study on the effect of exogenous additives on the fermentation process of Grifola frondosa is needed.
作者
雷露
吴天祥
王川南
LEI Lu;WU Tian-Xiang;WANG Chuan-Nan(School of Liquor and Food Engineering,Guizhou University,Guiyang,Guizhou 550025,China;Department of Food Engineering,Guizhou Vocational College of Foodstuff Engineering,Guiyang,Guizhou 551400,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2020年第10期1920-1932,共13页
Mycosystema
基金
国家自然科学基金地区科学基金项目(31460537)。
关键词
灰树花
天麻苦荞复配液
超高效液相色谱-四级杆串联飞行时间质谱
差异代谢物
Grifola frondosa
Gastrodia elata and Fagopyrum tataricum compound solution
ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS)
differential metabolite
