摘要
与钠皂和钾皂相比,在脂肪酸表面活性剂中使用胆碱作为抗衡离子可大大提高其水溶性,使长链脂肪酸衍生物在环境温度下得到应用。虽然胆碱已证实具有高生物相容性和低生物毒性,但根据欧洲化妆品管理规范76/768/EEC,所有化妆品均禁止使用胆碱盐。为了促进其应用,根据OCDE 301F标准研究了烷基链长为m=12~18的胆碱脂肪酸皂(ChCm)的生物降解性。对于奇数碳和偶数碳脂肪酸,测定了m=8~16的ChCm表面活性剂的细胞毒性。此外,为了更好与普通肥皂比较,并阐明阳离子的影响,还研究了钠和钾的同系物,揭示了疏水链长度与IC50(半抑制浓度)值之间的非线性关系。重要的是,实验数据表明ChCm表面活性剂的IC50值与广泛使用的钠皂和钾皂的IC50值一致。这表明胆碱羧酸盐表面活性剂是无害的,证明了其在终端消费品中的应用潜能。
Using choline as a counterion in fatty acid surfactants substantially increases their water solubility as compared to classical sodium and potassium soaps,and thereby enables the application of desirable longer-chain derivatives at ambient temperature.Choline is considered to be highly biocompatible and low toxic impact.However,according to the European Cosmetic Directive 76/768/EEC,all salts of choline are forbidden in cosmetic products,mainly just due to its classification as a quaternary ammonium ion.In order to facilitate their application in the future,we have investigated the biodegradability of choline soaps(ChCm)with alkyl chain lengths of m=12-18 according to the OCDE 301F standard.Further,the cytotoxicity of ChCm surfactants with m=8-16 was determined,both for odd-and even-numbered fatty acids.Studies were carried out using two different human cell lines,namely cervix carcinoma cells(HeLa)and keratinocytes(SK-Mel-28).For a better comparability to common soaps and to shed light on the influence of the cation,sodium and potassium homologues were also investigated.Results reveal an unexpected non-linear relationship between the hydrophobic chain length and the IC50 value.Most importantly,the presented data show that IC50 values of ChCm surfactants coincide with those of the widely applied sodium and potassium soaps.This demonstrates that choline carboxylate surfactants are harmless and thus strongly supports their applicability in end consumer products.
出处
《中国洗涤用品工业》
2020年第10期35-42,共8页
China Cleaning Industry
关键词
胆碱
胆碱脂肪酸皂
细胞毒性
生物降解力
choline
choline fatty acid soap
cell toxicity
biodegradability
