摘要
目的探讨卵巢浆液性腺癌组织中超长链脂肪酸延伸酶6(ELOVL6)及锌指转录调节蛋白4(KLF4)的表达水平及其与预后的关系。方法选取2012年5月至2014年5月青海红十字医院诊治的105例卵巢浆液性腺癌患者作为研究对象。其中高级别患者设为高级别组(n=75),低级别患者设为低级别组(n=30)。另选择因其他疾病行手术切除的正常卵巢组织和输卵管组织的患者设为对照组(n=25)。采用免疫组化法检测患者组织中ELOVL6及KLF4蛋白阳性表达,使用Spearman分析相关性,患者5年生存率采用Kaplan-Meier单因素分析,影响患者预后的独立危险因素采用Cox多因素分析。结果ELOVL6和KLF4蛋白在低级别组患者中的阳性表达率(53.33%、30.00%)显著低于对照组卵巢组织的阳性率(80.00%、64.00%),差异具有统计学意义(P<0.05),在高级别组患者中的阳性表达率(42.67%、16.00%)显著低于对照组输卵管组织的阳性率(88.00%、80.00%),差异具有统计学意义(P<0.05);高级别组患者Ⅲ期+Ⅳ期中ELOVL6和KLF4蛋白阳性表达率显著低于Ⅰ期+Ⅱ期,术后有残留灶患者的KLF4蛋白阳性表达率显著降低,差异具有统计学意义(P<0.05);高级别组患者ELOVL6和KLF4阳性表达率呈正相关,差异具有统计学意义(r=0.041,P=0.035);高级别组患者的ELOVL6蛋白阳性者5年生存率为40.6%,显著高于阴性者的21.8%,KLF4蛋白阳性者5年生存率为39.2%,显著高于阴性者的18.9%;Cox多因素分析显示KLF4低表达、FIGO分期均是影响高级别组患者预后的独立危险因素。结论卵巢浆液性腺癌患者组织中ELOVL6及KLF4蛋白低表达,FIGO分期和KLF4对患者预后有一定影响。
Objective To investigate the expressions of elongation of very long-chain fatty acids family member 6(ELOVL6) and Kruppel-like factor 4(KLF4) in ovarian serous adenocarcinoma and their relationships with the prognosis. Methods 105 patients with ovarian serous adenocarcinoma treated in Qinghai Red Cross hospital from May 2012 to May 2014 were selected, including 75 high-grade patients and 30 low-grade patients. 25 patients with normal ovarian tissue and fallopian tube tissue that were surgically removed due to other diseases were selected as control group. The positive expressions of ELOVL6 and KLF4 proteins were detected by immunohistochemistry. Spearman was used for correlation analysis;Kaplan-Meier univariate analysis was used to analyze patients′ 5-year survival rate;Cox multivariate analysis was used to analyze independent risk factors affecting the prognosis of patients. Results Immunohistochemical detection showed that the positive rates of ELOVL6 and KLF4 in low-grade patients(53.33%, 30.00%) were significantly lower than those in normal ovarian tissues(80.00%, 64.00%), with statistically significant differences(P<0.05), and those in high-grade patients(42.67%, 16.00%) were significantly lower than those in normal salpinx tissues(88.00%, 80.00%), with statistically significant differences(P<0.05);the positive expression rates of ELOVL6 and KLF4 proteins in high-grade patients of stage Ⅲ+Ⅳ were significantly lower than those in patients of stage Ⅰ+Ⅱ, and the positive expression rate of KLF4 protein in patients with residual lesions after operation were significantly lower, with statistically significant differences(P <0.05). There was no significant correlation between the expressions of ELOVL6 and KLF4 with omental metastasis and age;there was a positive correlation between ELOVL6 and KLF4 positive expression rate in high-grade patients, with statistically significant differences(r=0.041, P=0.035). In high-grade patients, the 5-year survival rate of ELOVL6 positive patients(40.6%) was significant
作者
李海鹏
LI Haipeng(Department of Obstetrics and Gynecology,Qinghai Red Cross Hospital,Xining 810000,Qinghai,China)
出处
《中国性科学》
2020年第10期39-43,共5页
Chinese Journal of Human Sexuality
关键词
卵巢浆液性腺癌
ELOVL6
KLF4
预后
Ovarian serous adenocarcinoma
Elongation of very long-chain fatty acids family member 6(ELOVL6)
Kruppel-like factor 4(KLF4)
Prognosis
