摘要
目的探讨乙醛脱氢酶2(ALDH2)rs671单核苷酸多态性(SNP)个体在RNA水平发生的改变。方法收集贵州医科大学附属医院体检者的外周抗凝全血48份。分别以人外周抗凝全血白细胞的DNA和互补脱氧核糖核酸(cDNA)为模板进行聚合酶链反应(PCR)扩增,然后对PCR产物进行Sanger测序鉴定受试者的ALDH2 rs671SNP位点和对应的cDNA的序列。结果48例受试者ALDH2 rs671(GG、GA、AA)3种基因型的基因频率分别为58.3%、39.6%和2.1%,并且所有受试者ALDH2 rs671SNP位点对应的cDNA的序列一致,都表现为“G”。结论48例受试者中,基因型表现为罕见的ALDH2 rs671(AA)的个体ALDH2 mRNA可能发生(A→G)RNA编辑。
Objective To investigate the changes of individuals with acetaldehyde dehydrogenase 2(ALDH2)rs671 single nucleotide polymorphism(SNP)at RNA level.Methods A total of 48 samples of peripheral anticoagulant whole blood were collected from the physical examination population in the Affiliated Hospital of Guizhou Medical University.The DNA and complementary DNA(cDNA)of human anticoagulant whole blood leukocyte was amplified by polymerase chain reaction(PCR),and then the PCR product was Sanger sequenced to identify the ALDH2 rs671SNP site and the corresponding cDNA sequence.Results The gene frequencies of the three genotypes of ALDH2 rs671(GG,GA,AA)in 48 subjects were 58.3%,39.6%and 2.1%respectively,and the sequence of ALDH2 rs671SNP site corresponding cDNA was the same in all subjects,which expressed"G".Conclusion In the 48 subjects we studied,individuals with rare genotypes of ALDH2 rs671(AA)occured RNA editing(A to G)in ALDH2 mRNA.
作者
夏万松
夏英
韦四喜
周春欢
杜洪
袁婷
金泳
黄海
XIA Wansong;XIA Ying;WEI Sixi;ZHOU Chunhuan;DU Hong;YUAN Ting;JIN Yong;HUANG Hai(School of Clinical Laboratory Science,Guizhou Medical University,Guiyang,Guizhou 550004,China;Department of Clinical Laboratory,the First Hospital Affiliated to Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550001,China;Center for Clinical Laboratory,the Affiliated Hospital of Guizhou Medical University,Guiyang,Guizhou 550004,China;Department of Clinical Laboratory,Guiyang Hospital of Guizhou Aviation Industry Group,Guiyang,Guizhou 550009,China)
出处
《国际检验医学杂志》
CAS
2020年第22期2693-2696,2700,共5页
International Journal of Laboratory Medicine
基金
国家自然科学基金项目(81760429)
贵州省科技计划项目[黔科合平台人才(2019)5610]
贵州省卫生和计划生育委员会科学技术基金项目(gzwjkj2018-1-072)
贵阳中医学院科研项目[(2018)101]
贵阳市科技计划项目[筑科合同(2019)9-2号]。
