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贮藏条件对江蓠琼胶酶活性和琼胶凝胶强度的影响 被引量:3

Effects of Storage Temperature and Humidity on Enzyme Activities and Strength of Agar Gel in Brown Alga Gracilaria
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摘要 通过调控保鲜盒中湿度获得水分为10%、14%、40%、60%的江蓠样品,然后将各组江蓠样品密封保鲜盒分别置于5、15、25℃和35℃恒温培养箱中,模拟贮藏温度和湿度环境,连续处理4 d,每日测定处理后江蓠中琼胶酶活性、还原糖含量、琼胶提取得率、琼胶凝胶强度等指标,连续测定4 d、每组3次。试验结果表明,环境温度和湿度的变化对江蓠琼胶酶活性和所提取的琼胶凝胶强度具有重要的影响,而湿度是最关键的影响因素,江蓠琼胶酶活性和琼胶的凝胶强度随着湿度的上升呈升高趋势。在贮藏开始1 d和2 d后,在江蓠水分含量为10%时提取琼胶的凝胶强度普遍高于水分含量为60%的江蓠所提取琼胶的凝胶强度,其胶凝胶强度最高达到604.2±136.7。 Brown alga Gracilaria samples were disposed in a fresh-keeping box and thus had moisture of 10%,14%,40%,and 60%by adjusting the humidity in the fresh-keeping box.Then the samples with different moistures were continuously treated in a constant temperature incubator at 5℃,15℃,25℃,and 35℃for 4 days,and agarase enzyme activity,reducing sugar content,agar extract extraction yield,agar gel strength,and other indicators were measured daily after treatment,three times in each group to clarify the intrinsic relationship between agarase activity and agar gel strength.The results showed that the changes in ambient temperature and humidity led to have an important influence on the activity of agaric enzyme and the strength of the extracted agar gel,with humidity being the most important factor.There were increases in agarase activity and the gel strength of agar as the humidity was elevated in the brown alga.There was stronger gel strength in the agar extracted from the brown alga containing 10%moisture 1 day and 2 days after the start of storage,with the maximum gel strength of(604.2±136.7),than that in gel extracted from the brown alga containing moisture of 60%.
作者 张曦文 伍菱 童风景 姜泽东 朱艳冰 肖安风 倪辉 李清彪 ZHANG Xiwen;WU Ling;TONG Fengjing;JIANG Zedong;ZHU Yanbing;XIAO Anfeng;NI Hui;LI Qingbiao(College of Food and Biological Engineering, Jimei University, Xiamen 361021, China;Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Technology, Xiamen 361021, China;Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed, Xiamen Southern Ocean Technology Center of China, Xiamen 361021, China;Research Center of Food Biotechnology of Xiamen City, Xiamen 361021, China)
出处 《水产科学》 CAS CSCD 北大核心 2020年第6期880-886,共7页 Fisheries Science
基金 国家自然科学基金资助项目(31401632,31501441) 海洋公益性行业专项基金资助项目(201505033).
关键词 江蓠 琼胶 还原糖 琼胶酶活性 凝胶强度 Gracilaria agar reducing sugar agarase activity gel strength
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