摘要
目的探讨白藜芦醇(RES)对肝部分切除大鼠肝组织自然杀伤(NK)细胞活性和肝再生的影响。方法随机将90只SD大鼠分为对照组、小剂量白藜芦醇和大剂量白藜芦醇处理组,各组30只,分别经尾静脉注射生理盐水、白藜芦醇10 g.kg^-1·d^-1和20 g.kg^-1·d^-1,连续5 d,然后行70%肝脏切除术。检测肝脏再生指数,使用流式细胞术测定肝组织NK细胞百分率,采用51 Cr释放法测定NK细胞杀伤活性,采用蛋白印迹法检测肝组织增殖细胞核抗原(PCNA)和CyclinD1蛋白表达。结果在24 h和48 h,大剂量白藜芦醇处理组大鼠肝脏再生指数分别为(1.89±0.04)和(2.45±0.07),显著大于小剂量白藜芦醇处理组[(1.59±0.06)和(2.12±0.05),P<0.05]或对照组[(1.43±0.03)和(1.92±0.05),P<0.05];大剂量白藜芦醇处理组大鼠NK细胞百分率分别为(24.62±1.36)%和(25.47±1.19)%,显著高于小剂量白藜芦醇处理组[(22.21±1.98)%和(22.36±1.78)%,P<0.05]或对照组[(17.36±1.78)%和(18.65±1.69)%,P<0.05];大剂量白藜芦醇处理组大鼠NK细胞杀伤活力分别为(48.48±2.69)%和(49.01±2.78)%,显著高于小剂量白藜芦醇处理组[(41.88±2.65)%和(42.32±2.58)%,P<0.05]或对照组[(28.32±2.36)%和(30.12±2.36)%,P<0.05];大剂量白藜芦醇处理组大鼠肝组织PCNA和CyclinD1蛋白相对表达量显著强于小剂量白藜芦醇处理组和对照组,差异有统计学意义(P<0.05)。结论RES可以促进肝部分切除术大鼠肝再生,可能与增强了NK细胞活性和相关蛋白表达有关。
Objective To explore the effects of resveratrol(RES)on liver regeneration(LR)and liver tissue naturacl killer(NK)cell cytoxicity in rats with partial hepatectomy.Methods 90 SD rats were randomly divided into control,low-dose of RES-intervened and high-dose of RES-intervened group,with 30 rats in each group,receiving normal saline,or RES at dose of 10 g.kg^-1·d^-1 or RES at dose of 20 g.kg^-1·d^-1 for five days.The 70%partial hepatectomy model was established thereafter.At 24 h and 48 h after surgery,15 rats in each group were sacrificed.LR index was detected.The percentage of liver tissue NK cells was determined by flow cytometry.The cytoxicity of liver NK cells was determined by 51 Cr release method and the Western blotting was applied to detect expression of PCNA and CyclinD1 protein.Results At 24 h and 48 h,the LR indexes in high-dose of RES group were(1.89±0.04)and(2.45±0.07),which were significantly higher than those in low-dose of RES group[(1.59±0.06)and(2.12±0.05),P<0.05]or in control group[(1.43±0.03)and(1.92±0.05),P<0.05];the percentages of NK cells were(24.62±1.36)%and(25.47±1.19)%,which were significantly higher than those in low-dose of RES group[(22.21±1.98)%and(22.36±1.78)%,P<0.05]or in control group[(17.36±1.78)%and(18.65±1.69)%,P<0.05];the cytoxicity activities of NK cells were(48.48±2.69)%and(49.01±2.78)%,which were significantly higher than those in low-dose of RES group[(41.88±2.65)%and(42.32±2.58)%,P<0.05]or in control group[(28.32±2.36)%and(30.12±2.36)%,P<0.05];the relative expression ofhepatic PCNA and CyclinD1 protein were significantly higher than those in low-dose of RES group or in the control group(P<0.05).Conclusion RES could promote NK cell proliferation and LR in rats after partial hepatectomy.
作者
李伟
郭春霞
刘苏南
Li Wei;Guo Chunxia;Liu Sunan(Department of Infectious Diseases,Union Hospital Affiliated to Huazhong University of Science and Technology,Wuhan 43000,Hubei Province,China)
出处
《实用肝脏病杂志》
CAS
2020年第6期785-788,共4页
Journal of Practical Hepatology
基金
湖北省自然科学基金资助项目(编号:02.07.040535)。
关键词
肝部分切除术
白藜芦醇
自然杀伤细胞
肝再生
大鼠
Partial hepatectomy
Resveratrol
Natural killer cell
Liver regeneration
Rats
