摘要
目的基于网络药理学对失笑散治疗原发性痛经的作用机制进行研究。方法通过TCMSP、BATMAN-TCM数据库结合OB、DL、HL检索失笑散中蒲黄(Pollen Typhae)及五灵脂(Faeces Trogopterpri)的化学成分和作用靶点,并通过Uniprot数据库将靶点校正为基因名,运用Genecards数据库获取原发性痛经的相关靶点与基因。借助Cytoscape3.6.1软件构建化合物靶点相互作用图,利用VENNY图将原发性痛经的靶点和药物靶点进行分析,筛选出二者共同靶点,应用STRING数据库构建蛋白相互作用网络图(PPI),通过David数据库对关键靶点进行GO富集分析及KEGG通路富集分析。结果筛选出失笑散治疗原发性痛经的12个有效成分,关键化合物为槲皮素和山奈酚;与治疗原发性痛经相关的靶点43个,关键靶点10个,主要靶点有IL-6、TNF、PTGS2、TP53、VEGFA等,10个关键靶点主要涉及细胞对缺氧的反应、肽基丝氨酸磷酸化的正向调节、炎症应答、NF-κB向核内输入的正调节等生物过程,通过参与肿瘤坏死因子信号通路、NOD样受体信号通路、NF-κB信号通路等参与治疗原发性痛经。结论本研究从多角度探索了失笑散治疗原发性痛经的可能作用机制,揭示了失笑散通过多成分、多靶点、多途径治疗原发性痛经,为其临床应用及进一步研究奠定了理论基础。
Objective To explore the mechanism of Shixiao Powder in treating primary dysmenorrhea based on network pharmacology.Methods The TCMSP,BATMAN-TCM databases combined with OB,DL and HL were used to retrieve the chemical components and targets of Pollen Typhae and Faeces Trogopterpri in Shixiao Powder.The targets were corrected to gene name through Uniprot database,and the Genecards database was used to obtain the relevant targets and genes of primary dysmenorrhea.With the help of Cytoscape3.6.1 software,the compound target interaction diagram was constructed,the drug target and primary dysmenorrhea target were analyzed by using VENNY map,and the common target of the two are screened out.Using STRING database to construct protein Interaction Network Diagram(PPI).Gene Ontology(GO)enrichment analysis and KEGG pathway enrichment analysis were carried out on key targets through David database.Results 12 main components of Shixiao Powder in treating primary dysmenorrhea were screened out.The most important compounds are quercetin and kaempferol.43 targets were related to primary dysmenorrhea,and 10 were key targets.The main targets are IL-6,TNF,PTGS2,TP53,VEGFA.The 10 key targets mainly involved in biological processes such as cell response to hypoxia,positive regulation of peptide serine phosphorylation,inflammatory response,positive regulation of NF-κB,etc.They participated in the treatment of primary dysmenorrhea through tumor necrosis factor signal pathway,NOD-like receptor signal pathway,NF-κB signaling pathway.Conclusion This study explored the possible mechanism of Shixiao Powder in the treatment of primary dysmenorrhea from multiple perspectives,revealing that Shixiao Powder acts through multi-component,multi-target and multi-pathway on primary dysmenorrhea.laying foundation for its clinicl and pharmacology study.
作者
胡国静
隋新月
王潇
刘卉
HU Guojing;SUI Xinyue;WANG Xiao;LIU Hui(The First Clinical Medical College of Shandong University of Traditional Chinese Medicine,Jinan 250014,China;Department of Gynecology,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China)
出处
《云南中医学院学报》
2020年第3期75-81,共7页
Journal of Yunnan University of Traditional Chinese Medicine
基金
山东省中医药科技发展计划(2017-071)。
关键词
失笑散
原发性痛经
网络药理学
Shixiao Powder
primary dysmenorrhea
network pharmacology
