摘要
以水稻OsNAC2过表达、RNAi转基因株系和野生型(日本晴)为材料,分别在苗期和生殖期进行干旱和盐胁迫处理,探索逆境条件下OsNAC2对水稻产量性状的影响。结果表明,不论是在苗期还是生殖期,OsNAC2-RNAi株系的叶相对含水量均比野生型更高,对干旱胁迫具有更强的适应能力;而OsNAC2过表达株系则相反。虽然苗期和生殖期遭遇盐胁迫的OsNAC2-RNAi株系相比野生型具有更高的叶相对含水量,但是OsNAC2的过量表达与RNAi株系的产量性状跟野生型相比并没有明显不同。生殖期干旱和盐胁迫下转基因株系的产量性状分析显示:干旱胁迫下,OsNAC2-RNAi株系的结实率与野生型相比显著提高了20.8%~29.2%,千粒重则无明显差异;而OsNAC2过表达株系每株粒数和千粒重相比野生型株系均显著降低。虽然盐胁迫下OsNAC2-RNAi株系的分蘖数和有效穗数明显比野生型高,但单株粒数和千粒重则无明显差异。上述结果表明,OsNAC2-RNAi株系具有更强的耐旱性,对于干旱胁迫下水稻的产量有显著的提高作用。
The OsNAC2-over expression,OsNAC2-RNAi and wild-type(Oryza sativa L.japonica.cv.Nipponbare)plants were used to study the effects of OsNAC2 on rice yield under drought and salt stresses during the seedling stage and reproductive stage respectively.The results showed that leaf relative water content of OsNAC2-RNAi plants was higher after drought stress during both of the seedling and reproductive stages,indicating that OsNAC2-RNAi plants exhibited higher tolerance to drought;meanwhile the performance of OsNAC2-OE plants was precisely in reverse.Although the leaf relative water content of OsNAC2-RNAi plants suffered from salt stress during both of the seedling and reproductive stages was higher than that of the wild-type plants,there was no obvious difference in rice yield among OsNAC2-OE,OsNAC2-RNAi and wild-type plants.The analysis of yield traits of transgenic lines during reproductive stage under drought or salt stresses showed that the seed setting rate of OsNAC2-RNAi lines was increased by 20.8%-29.2%more than that of wild-type plants after drought stress,with no significantly difference in 1000-grain weight.Further,the total grain number/plant and 1000-grain weight of OsNAC2-OE lines were significantly decreased compared with that of wild type lines.Although the number of tiller and effective panicle of OsNAC2-RNAi plants were significantly higher than those of wild-type plants under salt stress,there was no significant difference in grain number and 1000-grain weight per plant.These results suggested that OsNAC2-RNAi transgenic plants had more significant tolerance and higher yield under drought conditions.
作者
余江涛
李凌云
陈秋伶
钟群
梅雨辰
娄玉霞
金越
明凤
YU Jiangtao;LI Lingyun;CHEN Qiuling;ZHONG Qun;MEI Yuchen;LOU Yuxia;JIN Yue;MING Feng(College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;Development Center of Plant Germplasm Resources,Shanghai Normal University,Shanghai 200234,China)
出处
《上海师范大学学报（自然科学版）》
2020年第5期581-588,共8页
Journal of Shanghai Normal University(Natural Sciences)
基金
转基因生物新品种培育重大专项(2016ZX08009-001-008)
国家自然科学基金(31471152)。
