摘要
近年来,随着气候条件、耕作制度的变化和水肥条件的改善,小麦纹枯病在我国危害日趋严重,现已成为长江中下游麦区和黄淮麦区主要病害,每年造成严重经济损失[1]。小麦纹枯病常与小麦根茎部病害如根腐病、茎基腐病混合发生,发病早期症状相似,影响病害的早期诊断和防治。
Rhizoctonia cerealis is a soil-borne phytopathogenic fungus that causes wheat sharp eyespot,resulting in serious economic losses.In this study,the recombinase polymerase amplification combined with lateral-flow dipstick technology(Rc-RPA-LFD)was developed for the rapid and sensitivity detection of R.cerealis.The Rc-RPA-LFD assay could be completed at isothermal temperature of 38°C within 30 min without PCR thermal cyclers.The RPA primers and probe designed based on ITS region,showed high specificity to R.cerealis.The detection limit of Rc-RPA-LFD assay was 1 pg·μL-1 fungal genomic DNA,showed an equal sensitivity to that of conventional PCR.In addition,the Rc-RPA-LFD assay could detect R.cerealis from field soil samples,showing no significant differences compared to conventional PCR assay.The simplicity,rapidly and practicability all indicated that Rc-RPA-LFD assay will be a promising molecular diagnosis for the accurate and rapid detection of R.cerealis.
作者
鞠玉亮
沈鹏飞
冯艳娟
羊国根
刘文成
郭文华
潘月敏
JU Yu-liang;SHEN Peng-fei;FENG Yan-juan;YANG Guo-gen;LIU Wen-cheng;GUO Wen-hua;PAN Yue-min(Key Laboratory of Biology and Sustainable Management of Plant Disease and Pests of Anhui Higher Education Institutes,Innovation Team of Green Pesticide Development and Application in Anhui Province,Anhui Agricultural University,Hefei 230036,China;Agricultural Technology Promotion Center of Yongqiao District,Suzhou 234000,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2020年第5期618-621,共4页
Acta Phytopathologica Sinica
基金
国家重点研发计划子课题(2017YFD2021708)
安徽省科技重大专项(17030701050)。
