摘要
综述了脱氧核糖核酸酶(DNA酶)的起源及分离富集策略,对比了DNA酶与核糖核酸酶(RNA酶)及蛋白酶的相似点和不同之处,并重点讨论了产物捕获和对冲抵消等策略对筛选获得DNA酶的独到之处;同时系统回顾了近年来分离出的可特异感应各种金属离子或生物样本(包括细菌、细胞等),从而能在特定位点切割RNA底物的DNA酶探针;阐述了DNA酶领域现存的挑战,总结和展望了新思路和新方向.
In this review,we briefly describe the origin of deoxyribozymes and the selection strategies for the isolation of these deoxyribonucleic acid(DNA)enzymes.We compare deoxyribozymes with ribozymes and protein enzymes.We focus on the recently developed product-capturing and cancelling selection strategies,as well as the metal-ion-specific and particular-biological-sample-sensing ribonucleic acid(RNA)-cleaving deoxyribozymes which have worked as molecular tools in many aspects.In the end,we summarize the challenges for the deoxyribozyme field that need to be tackled down in the future.
作者
佟宗轩
胡沁沁
顾宏周
TONG Zongxuan;HU Qinqin;GU Hongzhou(Shanghai Institute of Cardiovascular Diseases,and Institutes of Biomedical Sciences,Zhongshan Hospital,Fudan University,Shanghai 200032,China)
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2020年第11期2345-2355,共11页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:21673050,91859104,81861138004)
国家重点研发计划项目(批准号:2016YFC1306400)资助.
