摘要
目的:建立草莓中矢车菊素-3-O-葡萄糖苷含量的测定方法。方法:以乙腈作为流动相A,1%磷酸水溶液作为流动相B进行梯度洗脱,流速0.5 mL/min,波长517 nm。结果:标准品的进样浓度在0.024 608~0.246 080μg呈良好的线性关系,相关系数r=1.000 0(n=7),加标回收率为100.18%,RSD为1.76%。结论:本文通过系统的方法学研究,建立了草莓中矢车菊素-3-O-葡萄糖苷的含量测定方法,所用方法简单,准确,重复性好,该方法可为不同品种的草莓质量控制提供可靠的依据。
Objective:To establish a method for the content determination of cyanidin-3-O-glucoside in strawberry.Methods:The content was determined by high performance liquid aqueous chromatography with acetonitrile as mobile phase A and 1%phosphoric acid aqueous solution as mobile phase B.The gradient elution was carried out at a flow rate of 0.5 mL/min and a wavelength of 517 nm.Result:There was a good linear relationship between 0.024608 and 0.246080μg.r=1.0000(n=7),the recovery rate of standard addition was 100.18%,and RSD was 1.76%.Conclusion:The method is simple,accurate and reproducible,could provide reliable basis for quality control of strawberry of different varieties.
作者
李飞鹤
韩波
佘梅
张俊英
Li Feihe;Han Bo;She Mei;Zhang Junying(Shanxi Zhendong Pharmaceutical Limited by Share Ltd,Taiyuan Shanxi 030001;Shanxi University of Chinese Medicine,Jinzhong Shanxi 030619)
出处
《山西中医药大学学报》
2020年第5期349-351,共3页
Journal of Shanxi University of Chinese Medicine
基金
山西省青年自然基金项目(201801D221433)。
