摘要
近年来土壤汞污染日益严峻,严重危害环境和人类健康,已成为一个世界性的环境问题,因此选择合理有效的方法对其进行修复与控制刻不容缓。有些微生物对汞具有吸附和挥发的特性,可用于受汞污染环境的修复。实验从受重金属污染的土壤中分离纯化出1株高抗汞真菌(DC-F9),其汞的最小抑菌浓度(MIC)达到160 mg/L;通过ITS序列比较,鉴别该菌属于曲霉属真菌;摇瓶培养发现,该真菌在Hg(Ⅱ)浓度为5 mg/L的培养基中对汞的挥发率、吸附率和总去除率分别为36.8%、58.4%、95.2%;而在Hg(Ⅱ)浓度为10 mg/L的培养基中,其值分别为45.4%、40.2%、85.6%;通过FTIR分析,发现该菌株为响应汞的胁迫表现出多种生理上变化。结果表明该菌株具有对汞污染环境修复的应用潜力。
In recent years,soil contaminated with mercury(Hg)has become serious problem on earth,endangering the environment and human health.Therefore,it is urgent to develop a reasonable and effective method to remediate it.Some microorganisms have capacity of adsorption and volatilization for Hg(Ⅱ),and can be applied to the bioremediation of Hg-contaminated environments.In this study,a highly Hg-resistant fungus(DC-F9)was isolated from the heavy metal-contaminated soil with a minimum inhibitory concentration(MIC)of 160 mg/L.The strain belonged to the genus Aspergillus by the phylogenetic analysis of its ITS sequence.In the medium supplemented with Hg(Ⅱ)concentration of 5 mg/L,the volatilization rate,adsorption rate and total removal rate of Hg(Ⅱ)were 36.8%,58.4%,95.2%respectively;and they were 45.4%,40.2%,85.6%respectively at Hg(Ⅱ)concentration of 10 mg/L.The strain exhibited a variety of physiological changes in response to mercury stress by FTIR analyses.These results showed that the DC-F9 had potentials of bioremediation for Hg-contaminated environment.
作者
司光正
杨清晨
董佳
闫天歌
常军军
陈金全
SI Guang-zheng;YANG Qing-chen;DONG Jia;YAN Tian-ge;CHANG Jun-jun;CHEN Jin-quan(Key Laboratory of Plateau Mountain Ecology and Degraded Environment Rehabilitation,College of Ecology and Environment,Yunnan University,Kunming 650091,China;Institute of International Rivers and Eco-Security,Yunnan University,Kunming 650091,China)
出处
《环境工程》
CAS
CSCD
北大核心
2020年第9期247-252,共6页
Environmental Engineering
基金
云南大学生态学与环境学院高原山地生态与退化环境修复重点实验室开放基金(2018DG005)
云南省教育厅基金(2019J0014)。
关键词
土壤汞污染
汞挥发
真菌
吸附
Hg-contaminated soil
volatilization
fungi
adsorption
