摘要
本文以牡蛎壳为钙源,制备L-天冬氨酸螯合钙,第一次较完整地探究其结构和理化特性。通过扫描电镜、红外光谱和质谱等技术表征螯合钙的结构,并对其物理化学特性进行研究。研究表明,螯合钙的微观形态整体呈团块状,结构较为紧密,表面有白色晶体吸附。红外光谱结果显示,L-天冬氨酸中的氨基和羧基主要以配位结合的形式参与了钙离子的螯合反应。一二级质谱结果证明,合成的螯合钙主要分子量响应峰值为342.9,其结构符合GB29226-2012中天门冬氨酸钙的结合方式。X射线衍射结果表明,所合成的L-天冬氨酸螯合钙为非晶态物质。相比于螯合反应前,螯合后的L-天冬氨酸螯合钙的热稳定性得到了一定程度的提高。在pH为5~8范围内的钙保留率均在90%以上,且具有良好的抗消化特性。本研究为牡蛎壳废弃物的高值化利用和开发牡蛎壳源的钙营养补充剂提供了理论基础和科学依据。
In this study,the oyster shell was used as the calcium source to prepare L-aspartate chelated calcium with its structure characterized and physicochemical property analyzed more completely for the first time.The structure of chelated calcium was characterized by scanning electron microscopy,Fourier transform infrared spectroscopy and mass spectrometry,and its physical and chemical properties were analyzed in many aspects.The results showed that the microscopic morphology of the chelated calcium was agglomerate.Its structure was firmer and its surface was adsorbed by white crystal.FT-IR result proved that the amino group and carboxyl group in L-aspartate were mainly participated in the chelation reaction of calcium ions in the form of coordination binding.The primary and secondary mass spectrometry results of the chelate indicated that the synthesized chelated calcium had a main molecular weight response peak of 342.9,and its structure conformed to the binding mode of calcium aspartate in GB29226—2012.In addition,in this study,we comprehensively studied the physicochemical characteristics of the chelated calcium crystal state,denaturation temperature,thermogravimetric mass loss and various stabilities and provided theoretical and scientific bases for the high value utilization of oyster shell waste and the development of calcium nutritional supplements for oyster shell.
作者
王真
姜岁岁
张帆
王润芳
冯雪
汪瑞
李诗洋
赵元晖
WANG Zhen;JIANG Sui-Sui;ZHANG Fan;WANG Run-Fang;FENG Xue;WANG Rui;LI Shi-Yang;ZHAO Yuan-Hui(College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China)
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第12期50-58,共9页
Periodical of Ocean University of China
基金
国家重点研究发展计划项目(2018YFD0901005)
山东省重点研究发展计划项目(2018GHY115012)
山东省自然科学基金项目(ZR2019MC014)资助。
关键词
牡蛎壳
L-天冬氨酸螯合钙
结构表征
理化特性
oyster shell
L-aspartate chelated calcium
structural characterization
physicochemical property
